CellVue Cell Labeling Kits for IR Fluorescent Cell Labeling
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CellVue® Cell Labeling Kits

CellVue fluorescent imaging kits use proprietary labeling technology to stably incorporate fluorescent dyes containing long aliphatic hydrocarbon tails into lipid membranes.1 They are useful for researchers working in all aspects of science and technology where fluorescently labeled cells and/or tissues are required. CellVue dyes also provide researchers with valuable tools for many in vivo and in vitro cell studies using fluorescent membrane labels.

The CellVue dyes are provided in an easy-to-use kit format containing a solution of the dye in ethanol and a cell labeling diluent. The labeling diluent provided with the kit is an iso-osmotic aqueous solution that contains no physiologic salts or buffers, detergents, or organic solvents, and is designed to maintain cell viability while maximizing dye solubility and staining efficiency.2,3 The simple membrane labeling protocol provided with the kit is applicable to almost any type of cell or organism.

fig1Overview

Figure 1. Two-color imaging of CellVue Burgundy and CellVue NIR815 stains with the Odyssey® Imager. A monocyte cell line (U937) was labeled with CellVue Burgundy (red), and a T-cell line (SUPT1) was labeled with CellVue NIR815 (green). The standard labeling protocol was used (1 x 107 cells/mL, 2 µM dye for 2-5 min at 25°C). Labeled cells were spotted on a glass surface (106 cells/spot), with the cell spots partially overlapping. Cell spots were imaged with the Odyssey Infrared Imager, in two distinct fluorescent channels. A) Monocytes labeled with CellVue Burgundy were detected in the 700 nm channel (red). B) T-cells labeled with CellVue NIR815 were detected in the 800 nm channel (green). C) Simultaneous imaging in both fluorescent channels clearly shows both cell populations (overlapping signals are shown in yellow). Signal bleed-through between the two channels was not observed. Images courtesy of S. van de Waarsenburg and D. Anderson, Burnet Institute, Melbourne, Australia.

LI-COR offers two CellVue Cell Labeling Kits:

    1. Horan, P. K., and Slezak, S. E., Nature, 340, 167-168 (1989).

    2. Horan, P. K., et al., Methods Cell Biol., 33, 469-490 (1990).

    3.Poon, R.Y., et al. In Living Color: Flow Cytometry and Cell Sorting Protocols, Diamond, R. A., and
    DeMaggio, S. (Eds.). p. 302-352 (Springer-Verlag, New York, 2000).


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