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CellTag 700 Stain ICW Kits provide detection reagents for cell-based In-Cell Western Assays. Each kit includes blocking buffer, IRDye® 800CW secondary antibody for detection of a specific protein target in the 800 nm channel, and CellTag 700 Stain to normalize well-to-well variations in cell number. This cost-effective normalization method makes quantification of the target protein more precise.
Detection of two proteins can be achieved using two different primary antibodies, followed by detection using two different secondary antibodies (one labeled with IRDye 800CW and the other with IRDye 680RD) in a multiplex assay. IRDye secondary antibodies are available for multiplex detection. Dilution factors and blocking conditions should be optimized for target and primary antibody combinations.