IRDye® Infrared Dyes
IRDye infrared fluorescent dyes and near-infrared (NIR) fluorescence imaging deliver enhanced sensitivity due to low background autofluorescence in the near-infrared region and, therefore, higher signal-to-noise ratios.
IRDye fluorescent dyes have absorption and emission wavelengths in the NIR spectrum, between 680 and 800 nm. Most of the IRDye reagents are compatible with the Odyssey® CLx, Odyssey Fc, Odyssey Sa, Pearl® Imagers, and 4300 DNA Analyzer platforms.
Table 1. IRDye Properties and Forms Available
|Dye||Exmax (nm)*||Emmax (nm)*||NHS ester||Maleimide||Carboxylate||DBCO||Azide||Alkyne||Phosphoramidite||Forms||LI-COR channel|
|IRDye 800CW||778||794||—||NHS Ester, Maleimide, Carboxylate, DBCO, Azide, Alkyne||800 nm|
|IRDye 800RS||770||786||custom||custom||custom||custom||custom||—||NHS Ester, custom||800 nm|
|IRDye 800 phosphoramidite||787||812||—||—||—||custom||custom||custom||DBCO, Azide, Alkyne, Phosphoramidite||800 nm|
|IRDye 750||766||776||custom||custom||custom||custom||—||NHS Ester, Maleimide, custom||—|
|IRDye 700DX||680||687||custom||custom||custom||custom||custom||—||NHS Ester, custom||700 nm|
|IRDye 700 phosphoramidite||680||697||—||—||—||—||—||—||DBCO, Azide, Alkyne, Phosphoramidite||700 nm|
|IRDye 680LT||680||694||custom||custom||custom||custom||—||NHS Ester, Maleimide, custom||700 nm|
|IRDye 680RD||680||694||—||NHS Ester, Maleimide, Carboxylate, DBCO, Azide, Alkyne||700 nm|
|IRDye 650||651||668||custom||—||NHS Ester, Maleimide, DBCO, Azide, Alkyne, custom||—|
*Data collected using methanol as the solvent.
**IRDye 680LT dye products should not be used for small animal in vivo imaging or In-Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
Many products from the IRDye Family of infrared dyes are being used in assays from other life science companies:
LI-COR Clinical Translational Research seeks to facilitate the use of IRDye 800CW imaging agents in clinical studies for detection of disease and its progression, and for monitoring of treatment and drug efficacy. Learn More.