IRDye® 680LT Applications
Figure 1. Immunofluorescence staining of HER2 protein localization to SK-BR-3 cell membrane. Cells were cultured on coverslips. After fixation and permeabilization, cells were stained with rabbit anti-HER2 mAb (Cell Signaling Technology), IRDye 680LT labeled goat anti-rabbit. Nuclei are stained with Sytox Green dye. Images were acquired with an Olympus microscope and deconvolved with the accompanying software.
Note: IRDye® 680LT dye products should not be used for small animal in vivo imaging or
In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.
Multiplex Western Blot Detection
Figure 2. Lysates of EGF-treated A431 cells were separated and transferred to nitrocellulose. The blot was probed with anti-ERK and anti-phospho-ERK primary antibodies, and then detected with IRDye 680LT and IRDye 800CW secondary antibodies. Blot was imaged with Odyssey® Fc System for 2 min. This phospho-ERK antibody crossreacts with phospho-EGFR (upper green band).