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IRDye® 680LT Labeling Kits

IRDye 680LT Protein Labeling Kits can be used to label peptides, antibodies and other proteins for applications such as Western blots and microscopy. These dyes and labeling kits are not recommended for labeling small animal imaging agents or for performing In-Cell Western™ assays.

Labeling Reactions

IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.

IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.

Note: IRDye® 680LT dye products should not be used for small animal in vivo imaging or
In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.

Table 1.

  Quantity of Protein to Label
  <100 µg 100 µg - 1 mg
Size of Protein

14-200 kDa

15-45 kDa

45-200 kDa

Detection Channel 800 nm 700 nm 800 nm 700 nm 800 nm 700 nm
  IRDye 800 CW - Microscale IRDye 680RD - Microscale
IRDye 680LT - Microscale
IRDye 800CW - Low MW IRDye 680RD - Low MW
IRDye 680LT - Low MW
IRDye 800CW - High MW IRDye 680RD - High MW
IRDye 680LT - High MW
High photostability needed? IRDye 700DX - Microscale IRDye 700DX - Low MW IRDye 700DX - High MW

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