Label Primary and Secondary Amines with IRDye 680LT Infrared Dye.
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IRDye® 680LT NHS Ester

The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

For sulfhydryl (cysteine-directed) labeling with maleimide dye, click here.

IRDye® 680LT in PBS

IRDye 680LT in PBS
Solvent Ext. Coeff. Abs Max Em Max
(M-1 cm-1) (nm) (nm)
Methanol 250,000 680 694
1X PBS 250,000 676 693
NHS ester reacts with a primary aliphatic amine, such as lysine.

NHS ester reacts with a primary aliphatic amine, such as lysine.

Dye label is attached through amide bond.

Dye label is attached through amide bond.


Note: IRDye® 680LT dye products should not be used for small animal in vivo imaging or
In‑Cell Western™ Assays. The higher level of fluorescent intensity creates high background making it unfavorable for use in these applications. We recommend using IRDye 680RD dye products for these applications.



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