IRDye 680RD Carboxylate is Non-Reactive and an Ideal Control.

IRDye® 680RD Carboxylate

Product Ordering Information

Order online or contact your local sales office.

Order online or contact your local sales office.

Order online or contact your local sales office.

To order, please contact your local distributor

Assays that use IRDye® 680RD conjugates (such as small animal imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

  • Carboxylate (non-reactive) form of IRDye 680RD is an ideal control.
    Note: The carboxylate dye has no reactive group and cannot be used for labeling.

How is IRDye 680RD carboxylate used?

In vitro cell-based assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

Small animal in vivo imaging

For evaluation of behavior and clearance of the dye itself

  • Timing of dye clearance from the animal's body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 680RD conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays


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