IRDye 680RD NHS Ester Used to Label Primary or Secondary Amines.
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IRDye® 680RD NHS Ester

The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

Absorption and Emission Spectra in 1X PBS

IRDye 680RD in PBS
Solvent Ext. Coeff. Abs Max Em Max
(M-1 cm-1) (nm) (nm)
Methanol 170,000 680 694
1X PBS 165,000 672 694
PBS:Methanol 165,000    

NHS ester reacts with a primary aliphatic amine, such as lysine.

NHS ester reacts with a primary aliphatic amine, such as lysine.

Dye label is attached through amide bond.

Dye label is attached through amide bond.



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