IRDye® 800CW Carboxylate
Product Ordering Information
IRDye® 800CW Carboxylate, 100 nmol [P/N 929-08972]
£150.00pack insert | msds/sds +wishlist
IRDye® 800CW Carboxylate, 5.0 mg [P/N 929-09406]
£611.00pack insert | msds/sds +wishlist
IRDye® 800CW Carboxylate, 50 mg [P/N 929-70122]
£1,847.00pack insert | msds/sds +wishlist
- Discounts available for quantities of 200 mg or more. Please contact firstname.lastname@example.org
Assays that use IRDye® 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.
Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.
Note: The carboxylate dye has no reactive group and cannot be used for labeling.
[ABOVE] Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly, and was completely cleared after 48 h.
Originally published in Kovar et al, Anal Biochem 367:1-12 (2007).
How is IRDye 800CW carboxylate used?
In vitro cell-based assays
As a control for cell-based assays that monitor binding of a dye-labeled agent.
- Validation of optical agents for in vivo administration
- Evaluation of binding specificity
In vivo imaging
For evaluation of behavior and clearance of the dye itself
- Timing of dye clearance from the animal's body
- Retention of dye in certain organs or sites (e.g., liver or kidneys)
Labeling Reaction Reference
As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.
Residual unreacted dye may cause:
- Artificially high values when dye/protein (D/P) ratio is calculated
- Increased background fluorescence in biological assays