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IRDye® 800CW Carboxylate

Assays that use IRDye® 800CW conjugates (such as in vivo imaging and cell binding assays) may require a "dye-only" control for potential effects or retention of the dye.

  • Carboxylate (non-reactive) form of IRDye 800CW is an ideal control.
    Note: The carboxylate dye has no reactive group and cannot be used for labeling.
Carboxylate clearance time course

[ABOVE] Clearance kinetics of IRDye 800CW carboxylate. A single SCID mouse was injected with 1 nmol of non-reactive IRDye 800CW dye, and clearance monitored over time as indicated. Pseudocolor fluorescence is superimposed on a white light image. IRDye 800CW carboxylate dispersed rapidly, and was completely cleared after 48 h.

Originally published in Kovar et al, Anal Biochem 367:1-12 (2007).


How is IRDye 800CW carboxylate used?

In vitro cell-based assays

As a control for cell-based assays that monitor binding of a dye-labeled agent.

  • Validation of optical agents for in vivo administration
  • Evaluation of binding specificity

In vivo imaging

For evaluation of behavior and clearance of the dye itself

  • Timing of dye clearance from the animal's body
  • Retention of dye in certain organs or sites (e.g., liver or kidneys)

Labeling Reaction Reference

As a standard to determine the amount of unreacted ("free") dye after IRDye 800CW conjugation and purification.

Residual unreacted dye may cause:

  • Artificially high values when dye/protein (D/P) ratio is calculated
  • Increased background fluorescence in biological assays


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