IRDye 800CW NHS Ester will Label Primary and Secondary Amines.
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IRDye® 800CW NHS Ester

The NHS ester reactive group will label primary and secondary amines, such as lysine residues in proteins.

IRDye 800CW in PBS
Solvent Ext. Coeff. (M-1cm-1) Abs Max (nm) Em Max (nm)
Methanol 300,000 778 794
Water 240,000 774 789
1X PBS 240,000 774 789
PBS: Methanol 270,000 777 791

NHS ester reacts with a primary aliphatic amine, such as lysine.

NHS ester reacts with a primary aliphatic amine, such as lysine.



Dye label is attached through amide bond.

Dye label is attached through amide bond.


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