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IRDye® QC-1 Infrared Dye

The first nonfluorescent (dark) quencher compatible with a wide range of visible and near-infrared fluorophores (~500 – 800 nm)

  • Used for FRET (fluorescence resonance energy transfer) applications such as protease assays
  • Efficient nonfluorescent quenching of near-infrared (NIR) dyes
  • Widest available quenching range – no need to carefully match the donor's fluorescence spectrum with the acceptor's absorbance
  • Pair IRDye QC-1 with any visible or near-infrared fluorophore within its wide wavelength range

IRDye QC-1 quencher can be used in protease assays.

  • Quenches common fluorophores with >97% efficiency1
IRDye QC-1 quencher can be used in protease assays.

Figure 1. Energy that is transferred from the donor (Dem) can be emitted at the acceptor's wavelength (Aem), quenching fluorescence.

1. Peng, X et al. A nonfluorescent, broad range quencher dye for Fõrster resonance energy transfer assays. Anal Biochem. 388(2):220-28 (2009)

IRDye QC-1 Absorption Spectrum in 1x PBS IRDye QC-1 Absorption Spectrum in Methanol
Solvent Ext. Coef. M-1cm-1 Absorption Max nm MW g/mole
1xPBS 96,000 737 1243.7
Methanol 98,000 788

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