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NHS Esters

Near-Infrared Fluorescence Labeling Reagents

IRDye® infrared dyes from LI-COR Biosciences are available for researchers developing applications using near-infrared (NIR) fluorescence. These unique dyes are used in a variety of NIR imaging applications including DNA sequencing, microsatellites, protein research, Western blotting, plate-based assays, and small animal imaging. Standard NHS ester chemistry is used to produce custom probes labeled with LI-COR IRDye infrared dyes. The NHS ester reactive group provides the functionality for labeling primary and secondary amino groups.

NHS Ester labeling cartoon

[Figure 1] NHS ester reacts with a primary aliphatic amine, such as lysine.

NHS Ester labeling cartoon

[Figure 2] Dye label is attached through amide bond.


If you are interested in commercial development rights using IRDye infrared dyes, please contact Business Development.


Dye Properties: Overview

Table 1. Data are for methanol only and are solvent-dependent. View dye properties for more information.

 
Extinction
Coefficient
Max. Abs.
(nm)
Max. Emis.
(nm)
Properties
IRDye 800CW
300,000
778
794
IRDye 680RD
170,000
680
694
IRDye 680LT
250,000
680
694
IRDye 750
330,000
766
776
IRDye 700DX
210,000
680
687
IRDye 800RS
300,000
770
786
IRDye 650
240,000
651
668


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