IRDye Protein Labeling Kits - LI-COR.

IRDye®Protein Labeling Kits

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IRDye Protein Labeling Kits

IRDye® Protein Labeling Kits label antibodies and other proteins for applications such as Western blots, In‑Cell Western™ assays, in vivo imaging, and whole organ or tissue section assays. IRDye labeling kits are cost-effective alternatives to expensive, time-consuming, custom antibody labeling services.

(Applications vary depending on the IRDye. Please refer to specific dye page.)

Labeling Reactions

IRDye reactive dyes bear an N-hydroxysuccinimide (NHS) reactive group that couples to free amino groups and forms a stable conjugate. Proteins other than IgG antibodies can be labeled, but protocol adjustment and optimization may be necessary.

IRDye labeling kits are based on a simple conjugation protocol that uses fast, spin column cleanup with resulting purity equivalent to purification by dialysis. Simply dissolve the protein in the provided buffer, combine it with the appropriate amount of water-soluble amine-reactive IRDye, and separate the labeled conjugate from the free dye using the included spin columns. Labeling and purification are complete in approximately 2 hours.


Table 1. Recommended IRDye Infrared Dyes for Labeling

Proteins Peptides Nucleic Acids
IRDye 800CW NHS Ester
IRDye 800RS NHS Ester
IRDye 700DX NHS Ester
IRDye 680LT NHS Ester
IRDye 680RD NHS Ester
IRDye 800 Phosphoramidite
IRDye 700 Phosphoramidite

Table 2.

Quantity of Protein to Label
<100 µg 100 µg - 1 mg
Size of Protein

14-200 kDa

15-45 kDa

45-200 kDa

Detection Channel 800 nm 700 nm 800 nm 700 nm 800 nm 700 nm
IRDye 800 CW
- Microscale
IRDye 680RD
- Microscale

IRDye 680LT


- Microscale
IRDye 800CW
- Low MW
IRDye 680RD
- Low MW

IRDye 680LT


- Low MW
IRDye 800CW
- High MW
IRDye 680RD
- High MW

IRDye 680LT


- High MW
High photostability needed? IRDye 700DX
- Microscale
IRDye 700DX
- Low MW
IRDye 700DX
- High MW


Product Ordering Information

Order online or contact your local sales office.

Order online or contact your local sales office.

Order online or contact your local sales office.

To order, please contact your local distributor


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