PVDF Membranes and Nitrocellulose Membranes for Westerns.
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Membranes and Membrane Kits

A low-background membrane is essential for infrared Western blot success. Background can be attributed to membrane autofluorescence or to detection of antibody non-specifically binding to the membrane. Polyvinylidene fluoride (PVDF) and nitrocellulose membranes are typically used for Western blotting applications. There are many brands and vendors available for both types of membrane.

Before any Western blot is performed on an Odyssey® System, the membrane of choice should be imaged "out of the box" on an Odyssey System to determine the level of autofluorescence. This is important because lot-to-lot variability occurs with some brands of PVDF. Membranes can be quickly evaluated by imaging them both wet and dry on any Odyssey System before use.

LI-COR has evaluated many different membranes for Western blotting; examples of membrane performance can be seen in Figure 1. There is typically more variability in PVDF membrane performance than for nitrocellulose membranes.

LI-COR offers high quality Immobilon®-FL PVDF, without lot-to-lot variability, packaged in affordable kits with the Odyssey Blocking Buffer or with 4X Protein Sample Loading Buffer. LI-COR also offers nitrocellulose membranes in both sheets and rolls.

For more information on factors affecting infrared Western blots, refer to our FAQs and Technical Tips.

fig1-membranes

Figure 1. Western blot detection of transferrin using various vendors and brands of PVDF membrane. PVDF membranes were scanned on the Odyssey Classic Infrared Imaging System in both 700 and 800 nm channels. LI-COR offers prescreened Immobilon-FL PVDF membranes in convenient, affordable kits.

Product P/N What’s Included
Odyssey® Nitrocellulose Membranes 926-31090 0.22 µm; ten 7 cm x 8.5 cm sheets
Odyssey Nitrocellulose Membranes 926-31092 0.22 µm; 30 cm x 3 m roll
Blocking Buffer (PBS) and PVDF Membrane Kit 926-31098 0.45 µm; 26.5 cm x 3.75 m roll + 500 mL Odyssey Blocking Buffer (PBS)
Blocking Buffer (TBS) + PVDF Membrane Kit 926-31099 0.45 µm; 26.5 cm x 3.75 m roll + 500 mL Odyssey Blocking Buffer (TBS)
4X Protein Sample Loading Buffer and PVDF Membrane Kit 926-31097 0.45 µm; 26.5 cm x 3.75 m roll + 15 mL 4X Protein Sample Loading Buffer
Bulk Blocking Buffer (PBS) and PVDF Membrane Kit 829-31080 0.45 µm; 26.5 cm x 3.75 m roll + 10 x 500 mL Odyssey Blocking Buffer (PBS)
Bulk Blocking Buffer (TBS) + PVDF Membrane Kit 926-31100 0.45 µm; 26.5 cm x 3.75 m roll + 10 x 500 mL Odyssey Blocking Buffer (TBS)


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