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Odyssey® Loading Indicator Workflow


Prepare samples

Step 1

Prepare samples and Odyssey® Loading Indicator (OLI).

Add OLI to samples, then heat

Step 2

Add OLI to samples, mix, and then heat the mixture.

Load equal volumes and perform electrophoresis

Step 3

Load equal volumes and perform electrophoresis.

Transfer to membrane

Step 4

Transfer to membrane.

Detect target proteins with IRDye

Step 5

Detect target proteins with IRDye® conjugated secondary antibodies.

Image membrane with an Odyssey imaging system

Step 6

Image membrane with an Odyssey imaging system in 700 nm or 800 nm channels.

Analyze blot with Image Studio

Step 7

Analyze blot with Image Studio™ Software.


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