PSVue® 794 Reagent Kit
Product Ordering Information
Figure 1. MPTP was used to induce cell death in mouse brains as a model for Parkinson's Disease. C57BI/6 mice were treated with MPTP to selectively destroy dopaminergic neurons. Mice were then injected with PSVue 794 dye or control dye and imaged on the Pearl® Imager 68 hrs post injection. A. control (i.e. non-targeting) dye; B. and C. PSVue 794 dye; D. excised brains from the three animals.
PSVue 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria and other anionic membranes. The PSVue 794 reagent kit contains components to provide a 1 mM solution of PSVue 794 in aqueous solution.
The compound exhibits fluorescence excitation maximum at 794nm and emission maximum at 810 nm and through its zinc(II)-dipicolylamine (Zn-DPA) moiety, it has been found to bind strongly to negatively charged bacterial cell walls (e.g. S. aureus, E. coli) and necrotic regions present in various tumors (e.g. mammary, prostate, glioma) in vitro and in vivo.
In particular, it has also been found to bind to the phosphatidylserine (PS) residues exposed on the cell surface of apoptotic cells, making it a more cost-effective alternative to fluorescently labeled Annexin V in various cell death assays. The labeling vehicle provided with the kit (Diluent X) is designed to maximize dye solubility and is suitable for in vitro and in vivo use.