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Sapphire700 Stain is a non-specific cell stain that accumulates in both the nucleus and cytoplasm of fixed or dead cells, but not live cells. When used to stain serial dilutions of A431 cells in 96-well plates, Sapphire700 Stain displays linearity of fluorescent signal for higher cell densities, from ~50,000 to ~250,000 cells/well.
Simultaneous staining of cells with Sapphire700 and DRAQ5® (Biostatus, Ltd.)* Stains expands the linear range, allowing more accurate normalization of cell number across both low and high cell densities.
*Can be purchased separately here