Choose the Right LI-COR Secondary Antibody for Your Application.
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Choosing the Right Dye-Conjugated Antibody

Select the right IRDye® or VRDye™ secondary antibody for your application using information in the table below. Here are some general guidelines to help you choose the right secondary antibodies:

  1. Choose IRDye 800CW secondary antibodies to get low background and high sensitivity in the 800nm channel. Use IRDye 800CW for targets where your require the highest sensitivity. Dilution working range 1:10,000 – 1:40,000.
  2. Choose IRDye 680RD secondary antibodies to get low background and for general use when detecting in the 700nm channel. Start using IRDye 680RD first over other 700 nm dyes. Dilution working range 1:10,000 – 1:40,000.
  3. Choose IRDye 680LT secondary antibodies to get high signal and for specific uses of detection in the 700nm channel. These antibodies are not recommended when getting up and running on system. Once established near-infrared protocols are optimized with IRDye 680RD, IRDye 680LT can be used to optimize signals in the 700 channel. Dilution range 1:20,000 – 1:40,000. Note: optimization may be required with IRDye 680LT. Please see pack insert for specific detergents/protocol modifications needed.

Application IRDye 800CW IRDye 680RD IRDye 680LT IRDye 650 VRDye 549 VRDye 490
Western Blot green_checkmark green_checkmark green_checkmark
In‑Cell Western™ Assay green_checkmark green_checkmark Not Recommended
On‑Cell Western Assay green_checkmark green_checkmark Not Recommended
Protein Array green_checkmark green_checkmark green_checkmark
Immunohistochemistry green_checkmark green_checkmark green_checkmark green_checkmark green_checkmark green_checkmark
Microscopy green_checkmark green_checkmark green_checkmark green_checkmark green_checkmark green_checkmark
Flow Cytometry green_checkmark green_checkmark green_checkmark
2D Gel Detection green_checkmark green_checkmark green_checkmark
Tissue Section Imaging green_checkmark green_checkmark green_checkmark
Virus Titration Assay green_checkmark
FRET-based Assays green_checkmark green_checkmark green_checkmark
Small Animal Imaging green_checkmark green_checkmark Not Recommended
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