VRDye 549 Secondary Antibodies for Visible Fluorescent Detection.
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VRDye 549 Secondary Antibodies

VRDye 549 secondary antibodies can be used for a variety of applications, including immunofluorescence microscopy, flow cytometry, and others. They are suitable for multiplex experiments when combined with other secondary antibodies labeled with proper fluorescent dyes and using instrumentation with appropriate excitation and detection capabilities.

Recommended for:

  • Microscopy
  • Immunohistochemistry
  • Flow Cytometry
VRDye 549 absorption and emmission in 1X PBS

​IHC staining of EGFR protein on F98-EGFR tumor slides. F98-EGFR tumors were snap-frozen in O.C.T.™ compound and sectioned at 4-μm thickness. After fixation and permeabilization, cells were incubated with rabbit anti-EGFR mAb (CST), followed by detection with VRDyeTM 549 Goat anti-Rabbit IgG (LI-COR P/N 926-54020). DAPI was used to stain the nuclei. Image acquired on Olympus IX81 microscope.

Complete VRDye 549 Secondary Antibody Listing

Host
Reactivity
Target Isotype
Application
Name Host Reactivity Target Isotype Applications Price


[P/N 926-54110]

Goat Mouse IgG IHC FC

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[P/N 926-54010]

Goat Mouse IgG IHC FC

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[P/N 926-54120]

Goat Rabbit IgG IHC FC

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[P/N 926-54020]

Goat Rabbit IgG IHC FC

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* Liquid 25 µL size is available in the US only.

Order online or contact your local sales office.

Order online or contact your local sales office.

Order online or contact your local sales office.

To order, please contact your local distributor



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