NewBlot^™ Stripping Buffer
for Nitrocellulose Membranes

NewBlot Nitro Stripping Buffer is specially formulated for nitrocellulose membranes.

• Ideal for removal of secondary antibody after detection of highly abundant targets. See performance table for stripping buffer comparisons.
• Optimized for stripping NIR Western blots detected with IRDye^® 800CW, IRDye 680LT, or IRDye 680RD secondary antibodies.


Figure 1. Example of stripping and reprobing with NewBlot Nitro Stripping Buffer. Rabbit anti-β-tubulin and mouse anti-ERK2 were run on a nitrocellulose membrane Western blot. The blot was probed with IRDye 680 Goat anti-Rabbit (red) and IRDye 800CW Goat anti-Mouse (green). The blot was stripped with NewBlot Nitro Stripping Buffer (1X) and reprobed three sequential times with the same antibodies. All blots have been scanned at 169 micron resolution and scan intensity of 6 on the Odyssey Infrared Imaging System.

• NewBlot Nitro should not be used with IRDye 700DX conjugates.

NewBlot Nitrocellulose Stripping Buffer may perform well with nitrocellulose membranes or antibody-dye conjugates from other manufacturers, but LI-COR neither guarantees nor supports such use.

Note: NewBlot Nitrocellulose Stripping Buffer is provided as a '5X' concentrated solution.