Product Ordering Information
NewBlot PVDF Stripping Buffer is formulated for use with PVDF membranes.
Figure 1. Example of stripping and reprobing with NewBlot PVDF Stripping Buffer. (A) Invitrogen EPAGE-96 6% gel loaded with replicate A431 lysate samples and detected with anti-Syk (IRDye 680, red bands) and anti-beta-Tubulin (IRDye 800CW, green bands). (B) Stripped with NewBlot-PVDF Stripping Buffer for 5 minutes at room temperature. (C) Blot was reprobed with anti-Syk and anti-beta-Tubulin antibodies. All blots have been scanned at 169 micron resolution and scan intensity of 6 on the Odyssey Infrared Imaging System.
NewBlot PVDF Stripping Buffer may perform well with PVDF membranes or antibody-dye conjugates from other manufacturers, but LI-COR neither guarantees nor supports such use.
Note: NewBlot PVDF Stripping Buffer is provided as a '5X' concentrated solution.