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NewBlot PVDF Stripping Buffer
for PVDF Membranes

Product Ordering Information

Order online or contact your local sales office.

Order online or contact your local sales office.

Order online or contact your local sales office.

To order, please contact your local distributor

NewBlot PVDF Stripping Buffer is formulated for use with PVDF membranes.

  • Ideal for removal of secondary antibody after detection of highly abundant targets. See performance table for stripping buffer comparisons.
  • Optimized for stripping NIR Western blots detected with IRDye 800CW, IRDye 680LT, or IRDye 680RD secondary antibodies.
  • Example of stripping and reprobing with NewBlot PVDF Stripping Buffer.

    Figure 1. Example of stripping and reprobing with NewBlot PVDF Stripping Buffer. (A) Invitrogen EPAGE-96 6% gel loaded with replicate A431 lysate samples and detected with anti-Syk (IRDye 680, red bands) and anti-beta-Tubulin (IRDye 800CW, green bands). (B) Stripped with NewBlot-PVDF Stripping Buffer for 5 minutes at room temperature. (C) Blot was reprobed with anti-Syk and anti-beta-Tubulin antibodies. All blots have been scanned at 169 micron resolution and scan intensity of 6 on the Odyssey Infrared Imaging System.

  • NewBlot PVDF Buffer should not be used with IRDye 700DX conjugates.

NewBlot PVDF Stripping Buffer may perform well with PVDF membranes or antibody-dye conjugates from other manufacturers, but LI-COR neither guarantees nor supports such use.

Note: NewBlot PVDF Stripping Buffer is provided as a '5X' concentrated solution.



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