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NewBlot Stripping Buffers

NewBlot Stripping Buffers are optimized for near-infrared (NIR) Western blots, including blots detected with IRDye® secondary antibodies.

The NewBlot family contains 3 different formulations:

  • NewBlot IR is a robust formulation that works with both nitrocellulose and PVDF membranes, and is a good place to start if you are new to stripping and reprobing NIR Western blots. NewBlot IR works well for removal of all IRDye secondary antibodies, including IRDye 800CW, 680RD, 680LT, 700DX, and may be suitable for removal of other dye-conjugated secondary antibodies. Additionally, it does not require hazardous shipping, unlike many other stripping buffers.

    NewBlot IR Stripping Buffer is not recommended for loading amounts over 30 µg.
  • NewBlot Nitro is intended for nitrocellulose membranes only, and is ideal for removal of secondary antibody.
  • NewBlot PVDF is intended for PVDF membranes only, and is ideal for removal of secondary antibody.
  • NewBlot Nitro and NewBlot PVDF are specially formulated for IRDye® secondary antibodies including IRDye 800CW, 680RD, and 680LT; but not 700DX.


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