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VRDye Protein Labeling Kits – High MW-Microscale

VRDye™ Protein Labeling Kits – High MW-Microscale are optimized to label primary or secondary antibodies for use with microscopy, flow cytometry, immunohistochemistry (IHC), and other applications where fluorophore-conjugated antibodies are required. These kits may also be used to label proteins spanning the molecular weight range of 50 kDa to 200 kDa.

The kits are optimized for labeling 100 µg of an IgG antibody. For larger amounts of antibody (1 mg), use the VRDye Protein Labeling Kits – High MW.

Each kit contains:

  • 3 Reactive Dye vials (0.15 mg) (store at -20 °C)
  • 0.5 mL 1 M Potassium Phosphate (K2HPO4), pH 9 (store at 4 °C)
  • 25 mL PBS (store at 4 °C)
  • 0.5 mL ultra pure water (store at 4 °C)
  • 3 Zeba™ Desalting Spin Columns (store at 4 °C)

    Note: The minimum recommended protein molecular weight for these columns is 7 kDa.

  • Pierce Zeba Desalting Spin Column instructions
  • Protocol for VRDye Protein Labeling Kit – High MW-Microscale
  • Quick Reference Guide

Refer to the kit pack insert for additional information on labeling proteins with the VRDye Protein Labeling Kits – High MW.



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