Odyssey® Western Blotting Kits
Figure 1. Dose response of EGFR phosphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to nitrocellulose. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye® 800CW Goat anti-Rabbit IgG (LI-COR P/N 926-32231) and IRDye 680RD Goat anti-Mouse IgG (LI-COR P/N 926-68070).
Reagents and Membranes for Quantitative Westerns
LI-COR® Odyssey Western Blotting Kits provide a convenient way to purchase reagents and membranes that are optimized for infrared fluorescence detection on the Odyssey Imaging Systems. The Western blotting kits provide enough secondary antibodies, blocking buffer, and membranes for 10 Western blots. Kits are available with either PVDF or nitrocellulose membranes and with either Odyssey Blocking Buffer (PBS) or Odyssey Blocking Buffer (TBS).
Infrared fluorescence detection has changed Western blotting (immunoblotting) by providing unparalleled quantification accuracy and simultaneous two-color multiplexed detection.
Odyssey Western Blotting Kits can be used for the following applications: