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Production of the Model 4300 has been discontinued as of January 1, 2014.
Service and support may become limited. Please contact your local sales office.


Computer System Requirements

  • Microprocessor:
    500 MHz Pentium II
  • Operating System:
    Windows® 95, 98, NT, 2000, XP, Vista
  • RAM:
    128 MB
  • LI-COR DNA Analyzer:
    Model 4300 or Model 4200.


  • Accessibility:
    e-Seq™ has secure communication (TCP/IP) with the 4300 through direct connection, network, or via the Internet.
  • Automated Lane Finding:
    e-Seq automatically finds lanes with high accuracy.
  • Base Calling:
    e-Seq features extended base calling with typical read lengths of 1000-1200 bases on 66 cm gels. Base calling is performed automatically using either a 'normal' or 'heterozygote' mode.
  • Quality Values:
    The e-Seq Software creates quality values at all base positions. Quality values for a sample can be viewed in a graph and exported directly to PHRAP or other analysis programs. Quality values can also be viewed side-by-side with curve and image data in various color-coded formats.
  • PHRED/PHRAP Compatibility:
    e-Seq automatically creates Phred-compatible curve files for base calling and quality value generation. The e-Seq base caller also produces a file comparable to PHRED (phd) that can be imported directly into PHRAP for assembly.
  • Image Display:
    Real time display of raw image data in a format similar to autoradiograms.
  • Post-Sequencing Actions:
    Following base calling, samples can be automatically transferred for further analysis. Examples of automated post-sequencing actions include:
    • Exporting sequencing data in various data bank file formats.

    • Exporting sample and curve files.

    • Exporting quality value tables.

    • E-mailing data in various formats.

    • Aligning and assembling sample files (requires AlignIR Software)

  • Multiple Instrument Control:
    A single computer with e-Seq Software can control any number of instruments. To start a run, e-Seq sends run parameters to the instrument. Server software in the 4300 takes over to finish the run. Since e-Seq is not needed during the run, the computer can be used for other tasks.
  • Data Formats:
    Available data formats for each sample include TIFF image, SCF curve, PHRED compatible curve, phd.1 file, quality value graph and numerous text formats compatible with standard databases.
  • Image and Sample Galleries:
    Galleries are used for organizing projects and editing data. Gallery functions allow projects containing numerous samples to be opened and viewed simultaneously. TIFF images produced using LI-COR Base ImagIRâ„¢ Software can also be imported into galleries for compatibility with previous LI-COR systems.
  • Sample Sheet Importing:
    Sample names can be imported in a number of standard file formats.

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