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Empiria Studio Software

Expert analysis made simple.

Created with high impact journals to get you reliable Western blot results

Publisher recommendations provide the best foundation for getting reproducible Western blot results. That’s why we developed Empiria Studio Software with the help of publishers.

When you use Empiria Studio to analyze Western blot images from your Odyssey® Imager, you will be confident that your analysis and results conform to accepted standards for reproducible Western blotting.

Validate, analyze, replicate, share

Empiria Studio is the only software that provides reliable answers at every step of the process.

With Empiria Studio, you have the ability to analyze validation blots, determine target protein expression, compare replicates, perform statistical analysis, and share your results using one software package.

Empiria Studio Software Workflow

Background on patent-pending background subtraction

Quantification in Empiria Studio uses a patent-pending background subtraction method optimized for reproducibility. We call it “Adaptive Background Subtraction” (ABS), because it adapts to the unique background in each image and subtracts background automatically.

Because the researcher does not choose a background subtraction method, variability caused by subjective background subtraction choices is eliminated.

Graph for Quantitative analysis with Empiria Studio was more reproducible than local and global background subtraction
Data image for Quantitative analysis with Empiria Studio was more reproducible than local and global background subtraction
Quantitative analysis with Empiria Studio was more reproducible than local and global background subtraction. Multiple users analyzed the designated bands (arrowheads on the image) using local, global/user-defined, and Empiria Studio Adaptive Background Subtraction. Variability of background-corrected band intensity, expressed as the mean % CV of the quantitative results for that image, was compared for each background subtraction method. In this figure from the Adaptive Background Subtraction White Paper, faint bands were surrounded by background in sample lanes. The graph shows the mean CV for each method (error bars show standard deviation, n = 6).

For more information, read the Adaptive Background Subtraction White Paper.

List of Experiments in Empiria Studio Software

Start strong and stay organized

Analysis with Empiria Studio starts simply — just create a Project. Project-based organization allows you to quickly find and share experimental data and related validation data. Empiria Studio will even remind you if your Project is missing validation data that publishers require.

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Validation is a great foundation

Empiria Studio has easy-to-follow analysis workflows for the validation experiments necessary to get reliable results.

Empiria Studio Software antibody validation screen

Antibody validation

Without an Antibody Validation, you can’t be sure your primary antibody is specific and selective to the expected target.

Empiria Studio Software combined linear range determination screen

Combined linear range determination

Without a Combined Linear Range Determination, you can’t be sure that you are accurately measuring changes in protein expression.

Empiria Studio Software antibody validation Housekeeping protein validation screen

Housekeeping protein validation

If you’re using a housekeeping protein as an internal loading control, you’ll want to make sure its expression isn’t altered by conditions in your experiment. So Empiria Studio has a workflow for Housekeeping Protein Validation, too.

Empiria Studio Software Calculations Screen

No more analysis paralysis

Once you’ve validated that your experimental design is robust, it’s time to run experiments and analyze your blots. With Empiria Studio, target protein data are automatically normalized (based on the type of internal loading control you used) and vital statistical values are automatically calculated.

At the end of a target analysis, Empiria Studio will calculate your fold change and automatically create a chart with the data. You can see results and confidently draw conclusions — using one software package. No more need for spreadsheets, graphing software, or word processing in your Western blot analysis and reporting process.

Replicate, replicate, replicate

Replicate samples and blots let you show that your results are robust and meaningful.

Empiria Studio lets you compare replicate samples on the same blot or across multiple blots to determine fold change, percent change, standard deviation, and other statistics. Now you can get the Western blot results you care about in one software package — without the uncertainty and hassle of formulas and spreadsheets!

Empiria Studio Software Scatter plot screen
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Empiria Studio Software publish and share illustration

Publish and share with all who care

No scientist is an island, so Empiria Studio makes it easy to share files and findings with your PI, colleagues, and publishers.

  • Export PDF reports with images, data tables, and graphs.
  • Export raw data as a CSV file or as an Excel spreadsheet.
  • Export blot images and graphs in the format publishers need.
  • Export all the contents of a Project — images, data, experimental details, results — in a single file that you can share with colleagues who have Empiria Studio.

Save on Your Empiria Studio License Today

Save money on Empiria Studio when you buy a license for yourself or your whole lab. Promotional price is available for a limited time only. Empiria Studio analyzes Quantitative Western blot images acquired on an Odyssey® Imager.

If you are currently a reviewer or part of the editorial staff at a scientific journal, Contact us to get your free license.

Empiria Studio Software Introductory Price
9141-500 1-User license $99.00 99.00 € £89.00
9141-505 5-User license $399.00 399.00 € £349.00
9141-510 25-User license $995.00 999.00 € £899.00

Empiria Studio requires a minimum of 8 GB of RAM; 64-bit versions of Windows 7 or 10; Mac OS Sierra or High Sierra.


Are you ready for reliable Western blot results today?

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