Software Designed for Western Blot Analysis
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No Longer a Fairy Tale

Free Software Designed for Western Analysis

Image Studio Lite

Image Studio Lite


Designed specifically for Western blot analysis, Image Studio™ Lite is simple, intuitive software. Its streamlined, ribbon-based design empowers you to quickly analyze and export publication-ready images. Best of all, it’s free and available to any researcher.

Over 50000 free downloads

Get Image Studio Lite

“You can sort your files based on parameters such as image date, the type of analysis, or the fluorescent color channels you used.”

Software for Image Analysis. Dance, A. (2016) The Scientist.

"The software is pretty intuitive to use, which makes it quite easy. It helps me a lot to acquire robust data quickly, also considering the background (which is a bit of a problem when using ImageJ, for instance)."

Elena Ilina of Edinger Institute / University Hospital Frankfurt via SelectScience® Reviews

“…Image Studio Lite provides additional data management features along with annotation tools for producing publication-ready images of your westerns.”

Analyzing gels and western blots with ImageJ. Miller, L. (2010). lukemiller.org.

Analyze Data Accurately

  • Import images in .jpg, .png, and .tif file formats
  • Adjust image display without changing your raw data

Eliminate Variability in Blot Quantification

  • Select bands quickly and quantify signal intensities
  • Subtract background consistently across your blot

Produce Publication-Ready Images

  • Annotate bands to produce publication-ready images
  • Export data for publication or create experiment reports for your colleagues

Optional Software Keys for Additional Analysis

Four analysis software keys are available for Image Studio Lite version 5.0 (or higher). If you need to update your software, download the latest version of Image Studio Lite.

In-Cell Western™ Key

Analyze in vitro assays in a microplate format with the In-Cell Western Key. For 96-well and 394-well microplates, circles are automatically placed over your wells. You can also perform ratiometric calculations.

Small Animal Imaging Key

Mark tumors, organs, and other regions of interest easily with the Auto Shape tool. Quickly isolate regions of interest with the pseudocolor display.

Plate/Grid Key

Place a pattern of shapes on an image for fast quantification.

Western Key

Analyze Western blot and multiplex (MPX™) Western blot images.

Computer Compatibility

At least 4 GB of RAM is required to run this magical software.

Windows® Operating Systems Compatibility

Image Studio Lite software Win8.1 Win8 Win7 XP Vista
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Mac® Operating Systems Compatibility

Image Studio Lite software 10.10 Yosemite 10.9 Mavericks 10.8 Mountain Lion 10.7 Lion 10.6 Leopard
5.x green checkmark green checkmark
4.0 green checkmark green checkmark
3.1 green checkmark green checkmark green checkmark
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