Highly-expressed “housekeeping” proteins include actin, tubulin, and GAPDH. When using this strategy, it’s important to validate each protein for your experimental conditions, including each tissue, treatment, and cell density, to ensure that their expression doesn’t change.
“‘House-keeping’ proteins should not be used for normalization without evidence that experimental manipulations do not affect their expression.”
Jensen et al. (2013) PLoS ONE 8(4): e61952.
Fluorescent detection of internal loading control
Fluorescent detection of target protein