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Probe Development Protocols and Resources

Over the past decade, LI-COR has developed and commercialized numerous IRDye conjugated probes and agents for molecular imaging applications. Through our dye technology, imaging platforms, and Custom Services, we provide comprehensive expertise to help you successfully take your imaging agents to clinic.

Quick Start Guides


Technical Notes for Probe Labeling and in vitro Imaging

Technical Notes for in vivo Imaging

Technical Notes and Protocols for ex vivo Imaging


  • Gong H, Kovar J, Little G, Chen H, Olive DM. (2010 Feb). In vivo imaging of xenograft tumors using an epidermal growth factor receptor-specific affibody molecule labeled with a near-infrared fluorophore. Neoplasia. 12(2):139-49.
  • Huang R, Vider J, Kovar JL, Olive DM, Mellinghoff IK, Mayer-Kuckuk P, Kircher MF, and Blasberg RG. (2012). Integrin αvβ3-Targeted IRDye 800CW Near-Infrared Imaging of Glioblastoma. Clin Cancer Res. 18(20): 5731–5740.
  • Kovar JL, Curtis E, Othman SF, Simpson MA, Olive DM. (2013 Sep 15). Characterization of IRDye 800CW chlorotoxin as a targeting agent for brain tumors. Anal Biochem. 440(2):212-9.
  • Kovar JL, Johnson MA, Volcheck WM, Chen J, and Simpson MA. (2006). Hyaluronidase Expression Induces Prostate Tumor Metastasis in an Orthotopic Mouse Model. Am J Pathol. 169(4): 1415–1426.
  • Kovar JL, Volcheck WM, Chen J, Simpson MA. (2007 Feb 1). Purification method directly influences effectiveness of an epidermal growth factor-coupled targeting agent for noninvasive tumor detection in mice. Anal Biochem. 361(1):47-54.
  • Kovar JL, Volcheck W, Sevick-Muraca E, Simpson MA, Olive DM. (2009 Jan 15). Characterization and performance of a near-infrared 2-deoxyglucose optical imaging agent for mouse cancer models. Anal Biochem. 384(2):254-62.
  • Kovar JL, Xu X, Draney D, Cupp A, Simpson MA, Olive DM. (2011 Sep 15). Near-infrared-labeled tetracycline derivative is an effective marker of bone deposition in mice. Anal Biochem. 416(2):167-73.
  • Kovar JL, Simpson MA, Schutz-Geschwender A, Olive DM. (2007 Aug 1). A systematic approach to the development of fluorescent contrast agents for optical imaging of mouse cancer models. Anal Biochem. 367(1):1-12.
  • Aguilar HN, Zielnik B, Tracey CN, Mitchell BF (2010) Quantification of Rapid Myosin Regulatory Light Chain Phosphorylation Using High-Throughput In-Cell Western Assays: Comparison to Western Immunoblots. PLOS ONE 5(4): e9965.
  • Huaxian Chen, Joy Kovar, Sean Sissons, Karen Cox, William Matter, Fred Chadwell, Peng Luan, Chris J. Vlahos, Amy Schutz-Geschwender, D. Michael Olive, A cell-based immunocytochemical assay for monitoring kinase signaling pathways and drug efficacy, Analytical Biochemistry, Volume 338, Issue 1, 2005, Pages 136-142, ISSN 0003-2697,

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