Casein Blocking Buffer in PBS

Casein Blocking Buffer for Western Blots and Other Assays.

Casein Blocking Buffer is a ready-to-use blocker formulation in phosphate-buffered saline (PBS) containing 0.1% sodium azide. It is stored at 4 °C.

Milk-based blockers may contain endogenous:

  • Biotin, which could interfere with biotin-streptavidin detection.
  • Phospho-proteins, which could interfere with phospho-specific antibodies.

Detection of p53 in A431 Lysates using Casein Blocking Buffer

Detection of p53 in serial dilutions of A431 lysate (5 μg to 78 ng). Casein was used for blocking and antibody dilution. Primary antibody: mouse anti-p53 (Sigma P/N P6874); secondary antibody: IRDye® 800CW Goat anti-Mouse (P/N 926-32210).

Selected P/N: 927-40200,927-40300

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