Chemi-IR Detection Kits

Chemi-IR Detection Kits Convert Chemiluminescent Westerns to NIR.

The Chemi-IR Detection Kit converts chemiluminescent Western blots to near-infrared fluorescent Western blot detection. Incubation of Western blots with IRDye® 800 Rabbit anti-HRP enables detection of HRP-conjugated antibody before or after chemiluminescent detection.

With the Chemi-IR Detection Kits, you can:

  • Use your existing HRP secondary antibodies
  • Detect on Odyssey® Infrared Imaging Systems
  • Eliminate substrates, film, and the darkroom
  • Make familiar chemiluminescent methods quantitative
  • Optimize your Western blot results

Kit Components

The 20 mini blot Kit (P/N 926-32234) contains:

  • Chemi-IR Conjugate (IRDye 800CW Rabbit anti-HRP, 0.1 mg, lyophilized)
  • Chemi-IR Diluent (100 mL)
  • Western Blot Incubation Box, Black, Large (11.5 x 8.8 x 2.8 cm)

The 100 mini blot Kit (P/N 926-32236) contains:

  • Chemi-IR Conjugate (IRDye 800CW Rabbit anti-HRP, 0.5 mg, lyophilized)
  • Chemi-IR Diluent (500 mL)
  • Western Blot Incubation Box, Black, Large (11.5 × 8.8 x 2.8 cm)

Linearity of the Chemi-IR Detection Kit

Serial dilutions of A431 lysates detected with the Chemi-IR detection kit
Integrated Intensity/A431 lysate (ng)
Serial dilutions of A431 lysates detected with the Chemi-IR Detection Kit. Serial dilutions of A431 lysates were run by SDS-PAGE and transferred to nitrocellulose. The blot was probed with monoclonal anti-p53 followed by Donkey anti-mouse HRP. The blot was detected with IRDye 800CW Rabbit Anti-HRP. The graph shows the Odyssey detection method is linear to at least 78ng of A431 lysate. Blot was quantified using Odyssey Imager analysis software.

Selected P/N: 926-32234,926-32236

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