IRDye® 650 Goat anti-Mouse IgG Secondary Antibody

IRDye 650 Goat anti-Mouse IgG Secondary Antibody

Immunogen

Mouse IgG paraproteins

Purity and Specificity

Isolation of specific antibodies was accomplished by affinity chromatography using pooled mouse IgG covalently linked to agarose. Based on ELISA and flow cytometry, this antibody reacts with the heavy and light chains of mouse IgG1, IgG2a, IgG2b, and IgG3, and with the light chains of mouse IgM and IgA. This antibody was tested by dot blot and and/or solid-phase adsorbed for minimal cross-reactivity with human, rabbit, goat, rat, and horse serum proteins, but may cross-react with immunoglobulins from other species. The conjugate has been specifically tested and qualified for immunofluorescence microscopy and flow cytometry applications.

Applications

  • Microscopy
  • Immunohistochemistry
  • Flow Cytometry

Formulation

IRDye 650 secondary antibodies are supplied as purified immunoglobulin conjugates, lyophilized in phosphate-buffered saline, pH 7.4. Protect from light. Store at 4 °C prior to reconstitution.

Each vial contains 10 mg/mL BSA (free of IgG and protease) as a stabilizer and 0.01% sodium azide as a preservative, after reconstitution. Concentration is 1.0 mg/mL when reconstituted as directed. Refer to the pack insert for details on reconstitution.

Recommended Dilutions

Application Recommended Suggested Range
Immunofluorescence Microsopy 1:400 1:100 - 1:1,000
Flow Cytometry 1:1,000 1:200 - 1:2,000
Other User-optimized

Suggested working dilutions are given as a guide only. Optimum dilutions will vary and should be determined empirically.

Selected P/N: 926-65010,926-65110

Price

Quantity

Add to Cart

+ Add to WishlistAdded. Go to Wishlist

IRDye 650 secondary antibodies are not suitable for use on Odyssey® Infrared Imaging Systems.

Resources

chat iconChat With Us
chat icon disabled