IRDye® 650 NHS Ester

IRDye 650 NHS Ester to Label Primary and Secondary Amines.

IRDye 650 NHS Ester is an infrared dye with detection near 650 nm. The NHS ester reactive group provides the functionality for labeling primary and secondary amines, such as lysine residues in proteins.

This dye is not suitable for use with LI-COR Odyssey® Imaging Systems.

IRDye® 800CW NHS Ester reaction
NHS Ester reacts with a primary aliphatic amine, such as lysine.
IRDye 800CW NHS Ester amide bond
Dye label is attached through amide bond.

Dye Structure

IRDye 650 NHS Ester Structure


  • Chemical Formula: C46H50N3Na3O16S4
  • Molecular Weight: 1098.12 g/mol
  • Exact Mass: 1097.18

Absorption and Emission Spectra

Solvent Ext. Coeff. (M-1cm-1) Absorption Maxima Emission Maxima
Methanol 240,000 651 nm 668 nm
1X PBS 230,000 648 nm 665 nm
PBS: Methanol 230,000

Absorption and Emission Spectra in 1X PBS

IRDye 650 Absorption and Emission Spectra

To discuss purchase quantities over 1 gram or commercial development rights using IRDye infrared dyes, please contact Business Development.

Selected P/N: 929-71012,929-71013



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