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IRDye® 650 Secondary Antibodies

Products

IRDye 650 secondary antibodies can be used for a variety of applications, including immunofluorescence microscopy, flow cytometry, and others. They are suitable for multiplex experiments when combined with other secondary antibodies labeled with proper fluorescent dyes and using instrumentation with appropriate excitation and detection capabilities.

Visible Fluorescence Reagents Overview

Recommended for:

  • Microscopy
  • Immunohistochemistry
  • Flow Cytometry

IRDye 650 Used for Immunofluorescence

Immunofluorescence staining of HER2 protein on SK-BR-3 cells. Cells were cultured on cover slips. After fixation and permeabilization, cells were incubated with rabbit anti-HER2 mAb (CST), followed by detection with IRDye 650 Goat anti-Rabbit (P/N 926-65020). Nuclei were stained with DAPI. Image acquired with Olympus IX81 microscope.
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