Use the Odyssey Chemifluorescent Substrate Kit (PVDF) to detect challenging targets on PVDF membranes.
With the Odyssey Chemifluorescent Substrate, you can get the benefits of near-infrared fluorescence detection with few changes to your chemiluminescent protocol. The substrate is oxidized by horseradish peroxidase (HRP) from the HRP-conjugated secondary antibodies, generating a precipitate that fluoresces and can be detected in the 700nm channel of Odyssey Imaging Systems.
When using chemifluorescent substrates, avoid using Odyssey blocking buffers or any other blocking buffers or antibody diluents that contain sodium azide, as it inhibits HRP activity.
Odyssey Chemifluorescent Substrate Kit (PVDF)
- 1 x 50 mL Odyssey Substrate Solution
- 1 x 50 mL Odyssey Oxidant Solution
- 10 Pre-cut Immobilon®-FL PVDF membranes, 10 cm x 10 cm each