Odyssey Western Blotting Kits for NIR Fluorescent Western Blots

Odyssey® Western Blotting Kits

Products

Western Blotting Kits with PVDF Membranes

Western Blotting Kits with Nitrocellulose Membranes

Odyssey Western Blotting Kits can be used for quantitative Western blots, In-Gel Westerns, or RNAi studies in which a phosphate-buffered saline (PBS) buffering system or a Tris-buffered saline (TBS) is used.

Convenient Kits for Multiplex Western Blots

These sample kits allow you to obtain a smaller quantity of infrared reagents and corresponding membranes to perform quantitative Western blots without committing to a large quantity of each.

  • Conveniently test LI-COR reagents and membranes for your Western blotting
  • Use near-infrared fluorescence detection on the Odyssey Imaging Systems
  • Experiment with multiplex Western blotting for simultaneous two-color detection

Kit Components

Each kit contains one IRDye® 800CW dye-labeled secondary antibody for the 800 nm channel, plus an additional secondary antibody labeled with either IRDye 680RD or IRDye 680LT for the 700 nm channel. Kits are available with either 10 Immobilon®-FL PVDF Membranes (0.45 μm, 10 cm x 10 cm) or 10 Odyssey Nitrocellulose Membranes (0.22 μm, 7 cm x 8.5 cm). All kits include Intercept® (PBS) Blocking Buffer or Intercept (TBS) Blocking Buffer.

Use Odyssey Western Blotting Kits for Multiplexing

Membrane Data
Dose response of EGFR phosphorylation in A431 cells. A431 cells were treated with serial dilutions of EGF (6.3-200 ng/mL). Lysates were resolved by SDS-PAGE and transferred to nitrocellulose. The blot was probed with rabbit anti-phosphoEGFR and mouse anti-ERK2 followed by detection with IRDye 800CW Goat anti-Rabbit IgG (P/N 926-32211) and IRDye 680RD Goat anti-Mouse IgG (P/N 926-68070).