PSVue 794 is a near-infrared fluorescent probe for detection of apoptotic and necrotic cells, bacteria, and other anionic membranes.
The compound has a fluorescence excitation maximum at 794 nm and emission maximum of 810 nm. Through its zinc(II)-dipicolylamine (Zn-DPA) moiety, PSVue 794 has been found to bind strongly to negatively charged bacterial cell walls (e.g., S. aureus, E. coli) and necrotic regions present in various tumors (e.g., mammary, prostate, glioma) in vitro and in vivo.
PSVue 794 has also been found to bind to the phosphatidylserine (PS) residues exposed on the cell surface of apoptotic cells, making it a more cost-effective alternative to fluorescently-labeled Annexin V in various cell death assays. The labeling buffer provided with the kit (Diluent X) is designed to maximize dye solubility and is suitable for in vitro and in vivo use.
Learn more about LI-COR optical probes.
- Pre-weighed amount of apo-PSS 794 solid dye, at least 1 mg
- Diluent X, 1 mL
- 4.2 mM Zinc Nitrate Solution in Diluent X, 1 mL
The PSVue 794 reagent kit contains components to provide a 1 mM solution of PSVue 794 in aqueous solution.
PSVue 794 Structure and Spectra
1.1 x 105 M-1 (in water)
0.14 in water (higher in organic solvent)
PSVue is a registered trademark of MTTI, used under license. PSVue is sold under license from MTTI. US Patent # 7,179,616 and others pending.