REVERT Total Protein Stain for Western Blot Normalization

REVERT Total Protein Stain for Western Blot Normalization

Make Western blot normalization more accurate and reliable with REVERT Total Protein Stain, a membrane-based (post-transfer) normalization strategy that stains all protein in your sample. REVERT Total Protein Stain works with your existing Western blot workflow and does not require special gels or equipment.

REVERT Total Protein Stain is a robust and replicable solution for Western blot normalization. The stain can be detected in the 700 nm channel of Odyssey® Imaging Systems (or competitor systems) for quantification and normalization analyses. The protocol is quick and can be completed within 15 minutes.

The 100 mL size is sufficient for 20 mini Western blots (5 mL/blot), and the 250 mL size is sufficient for 50 mini Western blots.

Accurate, Reliable Western Blot Normalization with REVERT Stain

REVERT Total Protein Stain works with your existing Western blot workflow, without needing any additional reagents or special gels. Staining takes less than ten minutes.

REVERT staining of PVDF and nitrocellulose membranes
Rapid staining with REVERT Total Protein Stain. REVERT Total Protein Stain provides highly efficient protein staining on nitrocellulose or Immobilon®-FL PVDF membranes in under 10 minutes. 10 µg of Jurkat cell lysate was loaded in each lane and separated in 4-12% NuPAGE Bis-Tris gels, transferred to nitrocellulose or PVDF membrane, and then imaged with the Odyssey CLx Imaging System in the 700 nm channel.

Selected P/N: 926-11011,926-11021

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