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DigiWest® High-Content Protein Profiling Services

LI-COR and NMI TT Pharmaservices have partnered to bring you DigiWest® Protein Profiling, a high-content, multiplex immunoassay performed as a contract research service. DigiWest® allows for the quick and efficient discovery of biomarkers or characterization of your potential targeted therapeutics. A DigiWest® assay provides you with comprehensive biological information deep into your chosen cell signaling pathway(s).

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The information gained from a DigiWest® Protein Profiling assay can help you determine which targets or pathways offer the most therapeutic promise. You can also use the assay to make predictions about the safety and toxicity of a therapeutic based upon functional effects and performance.

With DigiWest® Protein Profiling Services, you can get a head start in your drug development by focusing your attention on the most promising candidates. This helps ensure efficient use of your time, effort, and funds.

Watch to see how DigiWest® is making an impact in biomedical decision making and patient care.

Fast Results and Efficient Use of Your Sample

Only 20 to 60 μg of total protein are required to perform a DigiWest® assay. Using just 30 μg of snap-frozen tissue or dry-frozen cells, you receive comprehensive analyses using your choice of up to 800 different antibodies. Select from a library of over 1,200 prevalidated antibodies and over 50 predefined pathways available for human, mouse, rat, dog, minipig, and cynomolgus samples.

Uses for DigiWest® Protein Profiling Services

A DigiWest® immunoassay can provide data on several parameters useful in the development of targeted therapeutics.

  • Biomarker discovery to identify which biomarkers are promising targets for a therapeutic.
  • Lead characterization or mode-of-action study to provide insight into how a therapeutic works.
  • Predictive safety and toxicology investigation to estimate how safe a therapeutic might be based upon its functional effects.

Advantages of DigiWest® Protein Profiling

The analyses you receive from a DigiWest® Protein Profiling assay provide you with a more complete understanding of how your therapeutic may affect a target and creates a potential new starting point in your therapeutic development process.1 This can give you a head start on your therapeutic development by showing you which targets for treatment may or may not be effective.

  • High content
  • Low sample use
  • Library of over 1,200 prevalidated antibodies
  • 50+ predefined pathways
  • Targets total and phosphorylated proteins

The DigiWest® Assay Process

step 1

Proteins are size separated via SDS-PAGE.

step 2

Proteins are transferred to a blotting membrane and biotinylated.

step 3

Sample lanes are cut into 96 strips to generate 96 molecular weight fractions immobilized on membrane.

step 4

Proteins are eluted into 96-well plates.

step 5

Biotinylated proteins are immobilized on the surface of Neutravidin-coated Luminex beads. One distinct bead set is added to each of the 96 wells, which results in a collection of distinguishable protein-loaded bead sets from a single sample.

step 6

Beads are pooled to reconstruct the initial sample lane where a defined color code correlates with each molecular weight of the immobilized proteins.

step 7

Immunoassay: a small aliquot of the bead pool is incubated with Western blot antibodies before Phycoerythrin-labeled secondary antibodies are added for signal generation.

step 8

The sample is detected on a Luminex FLEXMAP 3D® instrument.

step 9

DigiWest® profile of protein analytes is generated.

Contact us to arrange a consultation with the DigiWest® Service Lab.

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References

  1. Kissel, M., Berndt, S., Fiebig, L., Kling, S., Ji, Q., Gu, Q., Lang, T., Hafner, F.T., Teufel, M., and Zopf, D. (2017). Antitumor effects of regorafenib and sorafenib in preclinical models of hepatocellular carcinoma. Oncotarget, 8(63), 107096–107108. https://doi.org/10.18632/oncotarget.22334

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