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Optoacoustic imaging, also known as photoacoustic imaging or multispectral optoacoustic tomography (MSOT), is an optical imaging modality that employs near-infrared light illumination for ultrasound detection. Near-infrared fluorophore IRDye 800CW and QC-1 (quencher) undergoes optoacoustic effect following exposure to an MSOT imager and allows ultrasound detection from tissue.
Pre-clinical biodistribution labeling therapeutic agents and theranostics with near-infrared dyes like IRDye 800CW enables assessment of biological distribution, uptake, localization, and specificity of the targeting molecules. For FRET applications and in vivo biochemical studies, dual labeling of the targeting molecules with a fluorophore and a quencher (like QC-1) greatly assists in imaging.
Targeting molecules conjugated to near-infrared dyes such as IRDye 800CW can aid surgeons by enhancing visualization during procedures. Fluorophores can also be used as imaging agents to improve contrast. Optical imaging can improve surgical outcomes in neurosurgery, angiography, lymph node mapping, and tumor resection.