COVID-19 Update: We remain open and ready to help. Read More


How to Maximize Sensitivity on Chemiluminescent Western Blots

Jeff Harford, Sr Product Manager for Imaging Systems at LI-COR, talks with one of our  advanced R&D scientists about how to maximize the sensitivity of chemiluminescent Western blot.

The discussion covers these factors and how they affect chemiluminescent Western blot sensitivity:

  • Choice of chemiluminescent substrate
  • Amount of substrate used
  • Placement of blot on film or imager
  • Freshness of your Western blot (i.e., time since the substrate was added)
  • Did the blot dry out before the chemiluminescent signal was captured?
  • Sequence of imaging methods - which should be performed first after substrate addition?
  • Length of acquisition time
  • Temperature of chemiluminescent substrate when used
  • Incubation time of blot with substrate
  • Substrate concentration

Learn more about the C-DiGit Chemiluminescent Western Blot Scanner and the Odyssey Fc Dual-Mode Imaging System.

chat iconChat With Us
chat icon disabled