Jeff Harford, Sr Product Manager for Imaging Systems at LI-COR, talks with one of our advanced R&D scientists about how to maximize the sensitivity of chemiluminescent Western blot.
The discussion covers these factors and how they affect chemiluminescent Western blot sensitivity:
- Choice of chemiluminescent substrate
- Amount of substrate used
- Placement of blot on film or imager
- Freshness of your Western blot (i.e., time since the substrate was added)
- Did the blot dry out before the chemiluminescent signal was captured?
- Sequence of imaging methods - which should be performed first after substrate addition?
- Length of acquisition time
- Temperature of chemiluminescent substrate when used
- Incubation time of blot with substrate
- Substrate concentration
Learn more about the C-DiGit Chemiluminescent Western Blot Scanner and the Odyssey Fc Dual-Mode Imaging System.