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What's New

We are constantly adding new products and new information to help you in your research. In addition, we update information to keep abreast of the latest innovations in life science research. Check this page often for the latest from LI-COR.

Products

Odyssey® Loading Indicators

Odyssey Loading Indicators (OLI) provide a simple, convenient method to evaluate the consistency of sample loading volume across gel lanes, as well as the uniformity of Western blot transfer. Learn more.

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REVERT™ Total Protein Stain

REVERT Total Protein Stain provides linear, proportional signal across a broad range of sample concentrations. Use this quick, total protein stain to correct for lane-to- lane variation in loading and transfer of target protein in your quantitative Western blot experiments.

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Odyssey Chemifluorescent Substrate Kits

With the Odyssey Chemifluorescent Substrate Kits, you can get the benefits of near-infrared fluorescence detection with few changes to your chemiluminescent protocol. Find out more.

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Featured Video

Normalization Methods for Quantitative Analysis of Western Blots

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LI-COR Press Release

LI-COR Sponsors Clinical Trial For Near Infrared Surgical Imaging

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Publication References

EMSA/Gel Shift Assay Publications

Traditional, radioactive EMSA protocols can be easily adapted to near-infrared fluorescence EMSA detection by using IRDye® end-labeled oligonucleotides and imaging with the Odyssey CLx or Classic Infrared Imaging System, providing a safe and sensitive alternative.

Application/Technical Notes

  • Determining the Linear Range for Quantitative Western Blot Detection

    Quantitative Western Blot analysis is accurate only if the target protein and internal loading control can both be detected in the same linear range. Read this protocol to find out how to determine this combined linear range.

  • Housekeeping Protein Validation

    Housekeeping proteins (HKPs) are routinely used for Western blot normalization. But before using an HKP for Western blot normalization, it is critical to validate that its expression is constant across all samples and unaffected by the specific experimental context and conditions. Read this protocol to understand how to do this.

  • Housekeeping Protein Normalization

    Expression of common housekeeping proteins is known to vary in response to certain experimental conditions. This protocol describes how to use a housekeeping protein for Western blot normalization and quantitative analysis.



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