Chlorophyll Fluorescence

Solar energy powers our ecosystem through the exquisite process of photosynthesis, which converts solar energy into chemical energy that is utilized by a series of enzymes to assimilate atmospheric CO2 into carbon skeletons used to build all organs of a plant, algae, etc. Infrared detection of CO2 and H2O gases is a means of quantifying CO2 assimilation, but this information directly pertains to only a portion of the photosynthetic process.

Fluorescence techniques have been developed to quantify the absorption and conversion of solar energy into the chemical energy used by the CO2 assimilatory reactions. Combining information from these independent measurements can provide critical information about how: 1) the CO2 and light absorption reactions are coupled; 2) plants tolerate various biological and environmental stresses; 3) light capture is regulated at the leaf level; and 4) all of these processes are impacted by genetic manipulation, a process that has resulted in increased yield of various species over the past several decades.

LI-COR's Solutions for Studying Chlorophyll Fluorescence:

The LI-6400XT and LI-6800 Portable Photosynthesis Systems combined with the Leaf Chamber Fluorometers allow the user to take simultaneous measurement of gas exchange and fluorescence over the same leaf area. The fluorometers are pulse-amplitude modulated (PAM) and can be used to take measurements on both dark- and light-adapted samples. Measured parameters include Fo, Fm, F, Fm', and Fo', and calculated parameters include Fv, Fv/Fm, dF/Fm, qP, qN, NPQ, and ETR. Both fluorometers support the Multiphase Flash™ Fluorescence (MPF) protocol to accurately estimate Fm’ at infinite irradiance.

The LI-6800 is our newest system, featuring a touch-screen interface, improved fluorometer, and much more. The 6800-01F MultiPhase Flash Fluorometer and Chamber delivers high intensity flashes over a larger leaf area, with greater uniformity. Uniform light during an induction event ensures that the exposed leaf area is responding to equal, unvarying illumination.

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