Poster Abstracts

Poster presenters showed 25 posters, featuring results from contemporary plant physiology, greenhouse gas, and agricultural research.

Workshops

Workshop presentations addressed leading research on landscape-level gas exchange, the physiology of photosynthesis, soil science, solar-induced chlorophyll fluorescence, and aquatic research.

Introduction to leaf-level gas exchange and fluorescence

Measurements of leaf-level gas exchange (carbon assimilation, apparent transpiration, stomatal conductance) and chlorophyll a fluorescence are fundamental to many areas of plant research. Techniques for making these measurements are well established and have been packed into off the shelf instrument systems. Successful use of these systems for collection of high-quality data, however, requires both an understanding of the underlying biological processes and the techniques used to measure them.

We will introduce the basic principles behind these measurements, with a focus on underlying assumptions and best practices.

CO2 response curves and fitting

Measurements of photosynthetic response to CO2 give insight into the limitations and capacities of photochemistry. We will demonstrate techniques for measuring CO2 response using both steady-state and non-steady-state approaches, and curve fitting in R to get parameter estimates (Vcmax and Jmax). We will discuss considerations for developing a protocol and configuring an instrument for these measurements.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Gas exchange from algal suspensions and other liquid samples

We will discuss the application of steady-state gas exchange techniques to liquid samples. Topics will include application examples in algal systems and general technical consideration for CO2 exchange measurements from liquid samples.

Stomatal conductance in needles and narrow leaves

We will focus on technological advances in the measurement of stomatal conductance from needle-like and narrow leaves. Topics will include application examples in pine (Pinus sp.) dominated forest systems and technical differences between various implementations of this measurement.

Light response curves and fitting

Measurements of photosynthetic response to light give insight to the limitations of photochemistry and light use. We will demonstrate techniques for measuring light response and curve fitting in R to get parameter estimates (Asat , Rd, apparent quantum yield…). We will discuss considerations for developing a protocol and configuring an instrument for these measurements.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Integrating leaf-level gas exchange and stable carbon isotope measurements

Online measurements of CO2 isotopologue discrimination, in parallel with carbon assimilation, provide a powerful tool to subsegment the diffusion path for CO2 across the leaf, allowing for estimation of mesophyll conductance and the CO2 concentration at the chloroplast. Historically, combined isotope gasexchange systems have been custom built laboratory instruments. Advances in laser-based analyzers and capabilities of off-the-shelf gas-exchange systems have opened up the possibility for more plug-and-play integration and instruments suitable for field use.

We will review the theory of the isotopic measurement, its applications, and discuss the technical aspects of integrating the current generation of laser-based analyzers with an existing gas-exchange system. We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Survey measurements

These will be held as two back-to-back sessions of approximately 20 minutes each. We will demonstrate techniques for using leaf-level gas-exchange instruments for survey style measurements. This will include discussion around selecting and configuring the chamber environment and general measurement best practices.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Introduction to chamber-based measurements of trace gas fluxes from soils

Trace gas transport (flux) at the soil-atmosphere interface is an important process linked to a number of biological and physical properties of an ecosystem. We will provide an introduction to the theory and application of closed-transient chambers to the measurement these fluxes. Topics will include diffusive transport theory, fitting methods for flux calculation and considerations for deploying chamber systems.

Stable carbon isotopes for chamber-based measurements

The bulk flux of CO2 from the soil is linked to multiple biological processes in and around the soil and physical properties across the soil-atmosphere continuum. Stable isotopologues of CO2 measured in conjunction with this flux can be used to better understand its drivers and carbon sources. This measurement has historically relied on manual sample collection and off-line analysis in the laboratory via mass spectrometry. The introduction of laserbased analyzers for isotopologue measurements has opened the opportunity for online field-based analysis, but integration of these analyzers with fielddeployed, chamber-based systems has proven difficult. We will provide an introduction to isotopologue measurements and applications, and discuss integration of a laser-based isotopologue analyzer with an off-the-shelf chamber system.

Greenhouse gas emissions: mobile detection and quantification

The location, attribution, and quantification of greenhouse gas (GHG) emissions is an important task in the reduction of the release of these gases into the atmosphere, and the mitigation of climate change. A better understanding of the sources, both natural and anthropogenic, of these gases is the first step towards this goal. Mobile, vehicle-based measurements of GHGs play a vital role in the survey of urban and rural environments, discriminating sources, and quantifying emissions from anthropogenic sources such as transport, oil & gas exploration and production, and intensive agriculture. We will describe the use of vehicle-based trace gas analyzers for mobile measurements of CH4, CO2, and N2O, delivering precise and accurate measurements with actionable spatial resolution.

SoilFluxPro™

SoilFluxPro™ is a freely available software for post processing and quality control of chamber-based trace gas flux measurements. We will provide an introduction to SoilFluxPro and its use. We will discuss differences in its various curving fitting approaches for flux estimates, its advanced guidance tools selecting fit windows, and its various other features, including its hidden scripting console.

Open SoilFluxPro™ session

We will tackle any theoretical or practical questions you may have about SoilFluxPro™. We will also welcome your suggestions for future developments.

Introduction to eddy covariance for decision-makers

This workshop offers senior business leaders a high-level understanding of the most direct and defensible method of emission measurements available to date, its history in academia, and its emerging power and advantages in various fields outside academia. It explores carbon sequestration, GHG emissions, water management, as well as climate change and carbon credit impacts. Leaders gain the knowledge needed for making informed decisions about sustainability and environmental management.

Introduction to eddy covariance methodology

An introduction to methodological aspects of the eddy covariance technique: theory, basic assumptions, key principles, fetch and flux footprint considerations,experimental design, and implementation.

Eddy covariance data processing theory–from raw data to fluxes

We will explore the theory behind calculations of eddy covariance fluxes from raw data, and the wealth of information that can be derived from raw eddy covariance data, including data quality, spectra, cospectra, and footprint estimations.

Eddy covariance in agriculture, crop, and water resource management, including a LI-710 performance review

We discuss agriculture-focused eddy covariance technique that provides essential insights into greenhouse gas emissions (GGE), carbon sequestration, evapotranspiration (ET), water use efficiency (WUE), crop management, and overall agricultural ecosystem productivity.

We also discuss a new mid-to-low-cost eddy covariance sensor for evapotraspiration (ET) measurements, LI-710, developed for practical decision-making applications and decisions support. This small plug-and-play ET sensor can be easily installed anywhere by a non-professional, and significantly reduces cost and power needs compared to traditional eddy covariance systems used for directly measuring ET.

The sensor directly measures evapotranspiration rates, sensible heat flux, temperature, humidity, atmospheric pressure and their derivatives from various environments like agricultural fields, wetlands, lakes, rivers, and reservoirs, providing pivotal data for water balance calculations, availability assessments, and irrigation management.

Eddy covariance data presentation, visualization, and evaluation

We will discuss ways of visually summarizing and presenting eddy covariance results, including spectra, cospectra, ogives, footprint estimations and allocation, wavelets and flux mapping. We will also discuss how to use those visualizations to evaluate the quality and significance of resulting fluxes.

Open EddyPro® session

We will tackle any theoretical or practical questions you may have about the EddyPro® eddy covariance flux computation software. We will also welcome your suggestions for future developments.

Introduction to leaf-level gas exchange and fluorescence

Measurements of leaf-level gas exchange (carbon assimilation, apparent transpiration, stomatal conductance) and chlorophyll a fluorescence are fundamental to many areas of plant research. Techniques for making these measurements are well established and have been packed into off the shelf instrument systems. Successful use of these systems for collection of high-quality data, however, requires both an understanding of the underlying biological processes and the techniques used to measure them.

We will introduce the basic principles behind these measurements, with a focus on underlying assumptions and best practices.

CO2 response curves and fitting

Measurements of photosynthetic response to CO2 give insight into the limitations and capacities of photochemistry. We will demonstrate techniques for measuring CO2 response using both steady-state and non-steady-state approaches, and curve fitting in R to get parameter estimates (Vcmax and Jmax). We will discuss considerations for developing a protocol and configuring an instrument for these measurements.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Gas exchange from algal suspensions and other liquid samples

We will discuss the application of steady-state gas exchange techniques to liquid samples. Topics will include application examples in algal systems and general technical consideration for CO2 exchange measurements from liquid samples.

Stomatal conductance in needles and narrow leaves

We will focus on technological advances in the measurement of stomatal conductance from needle-like and narrow leaves. Topics will include application examples in pine (Pinus sp.) dominated forest systems and technical differences between various implementations of this measurement.

Light response curves and fitting

Measurements of photosynthetic response to light give insight to the limitations of photochemistry and light use. We will demonstrate techniques for measuring light response and curve fitting in R to get parameter estimates (Asat , Rd, apparent quantum yield…). We will discuss considerations for developing a protocol and configuring an instrument for these measurements.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Integrating leaf-level gas exchange and stable carbon isotope measurements

Online measurements of CO2 isotopologue discrimination, in parallel with carbon assimilation, provide a powerful tool to subsegment the diffusion path for CO2 across the leaf, allowing for estimation of mesophyll conductance and the CO2 concentration at the chloroplast. Historically, combined isotope gasexchange systems have been custom built laboratory instruments. Advances in laser-based analyzers and capabilities of off-the-shelf gas-exchange systems have opened up the possibility for more plug-and-play integration and instruments suitable for field use.

We will review the theory of the isotopic measurement, its applications, and discuss the technical aspects of integrating the current generation of laser-based analyzers with an existing gas-exchange system. We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Survey measurements

These will be held as two back-to-back sessions of approximately 20 minutes each. We will demonstrate techniques for using leaf-level gas-exchange instruments for survey style measurements. This will include discussion around selecting and configuring the chamber environment and general measurement best practices.

We highly recommend that those new to gas-exchange measurements attend Introduction to leaf-level gas exchange and fluorescence before attending this workshop.

Introduction to chamber-based measurements of trace gas fluxes from soils

Trace gas transport (flux) at the soil-atmosphere interface is an important process linked to a number of biological and physical properties of an ecosystem. We will provide an introduction to the theory and application of closed-transient chambers to the measurement these fluxes. Topics will include diffusive transport theory, fitting methods for flux calculation and considerations for deploying chamber systems.

Stable carbon isotopes for chamber-based measurements

The bulk flux of CO2 from the soil is linked to multiple biological processes in and around the soil and physical properties across the soil-atmosphere continuum. Stable isotopologues of CO2 measured in conjunction with this flux can be used to better understand its drivers and carbon sources. This measurement has historically relied on manual sample collection and off-line analysis in the laboratory via mass spectrometry. The introduction of laserbased analyzers for isotopologue measurements has opened the opportunity for online field-based analysis, but integration of these analyzers with fielddeployed, chamber-based systems has proven difficult. We will provide an introduction to isotopologue measurements and applications, and discuss integration of a laser-based isotopologue analyzer with an off-the-shelf chamber system.

Greenhouse gas emissions: mobile detection and quantification

The location, attribution, and quantification of greenhouse gas (GHG) emissions is an important task in the reduction of the release of these gases into the atmosphere, and the mitigation of climate change. A better understanding of the sources, both natural and anthropogenic, of these gases is the first step towards this goal. Mobile, vehicle-based measurements of GHGs play a vital role in the survey of urban and rural environments, discriminating sources, and quantifying emissions from anthropogenic sources such as transport, oil & gas exploration and production, and intensive agriculture. We will describe the use of vehicle-based trace gas analyzers for mobile measurements of CH4, CO2, and N2O, delivering precise and accurate measurements with actionable spatial resolution.

SoilFluxPro™

SoilFluxPro™ is a freely available software for post processing and quality control of chamber-based trace gas flux measurements. We will provide an introduction to SoilFluxPro and its use. We will discuss differences in its various curving fitting approaches for flux estimates, its advanced guidance tools selecting fit windows, and its various other features, including its hidden scripting console.

Open SoilFluxPro™ session

We will tackle any theoretical or practical questions you may have about SoilFluxPro™. We will also welcome your suggestions for future developments.

Introduction to eddy covariance for decision-makers

This workshop offers senior business leaders a high-level understanding of the most direct and defensible method of emission measurements available to date, its history in academia, and its emerging power and advantages in various fields outside academia. It explores carbon sequestration, GHG emissions, water management, as well as climate change and carbon credit impacts. Leaders gain the knowledge needed for making informed decisions about sustainability and environmental management.

Introduction to eddy covariance methodology

An introduction to methodological aspects of the eddy covariance technique: theory, basic assumptions, key principles, fetch and flux footprint considerations,experimental design, and implementation.

Eddy covariance data processing theory–from raw data to fluxes

We will explore the theory behind calculations of eddy covariance fluxes from raw data, and the wealth of information that can be derived from raw eddy covariance data, including data quality, spectra, cospectra, and footprint estimations.

Eddy covariance in agriculture, crop, and water resource management, including a LI-710 performance review

We discuss agriculture-focused eddy covariance technique that provides essential insights into greenhouse gas emissions (GGE), carbon sequestration, evapotranspiration (ET), water use efficiency (WUE), crop management, and overall agricultural ecosystem productivity.

We also discuss a new mid-to-low-cost eddy covariance sensor for evapotraspiration (ET) measurements, LI-710, developed for practical decision-making applications and decisions support. This small plug-and-play ET sensor can be easily installed anywhere by a non-professional, and significantly reduces cost and power needs compared to traditional eddy covariance systems used for directly measuring ET.

The sensor directly measures evapotranspiration rates, sensible heat flux, temperature, humidity, atmospheric pressure and their derivatives from various environments like agricultural fields, wetlands, lakes, rivers, and reservoirs, providing pivotal data for water balance calculations, availability assessments, and irrigation management.

Eddy covariance data presentation, visualization, and evaluation

We will discuss ways of visually summarizing and presenting eddy covariance results, including spectra, cospectra, ogives, footprint estimations and allocation, wavelets and flux mapping. We will also discuss how to use those visualizations to evaluate the quality and significance of resulting fluxes.

Open EddyPro® session

We will tackle any theoretical or practical questions you may have about the EddyPro® eddy covariance flux computation software. We will also welcome your suggestions for future developments.

Agenda

See the agenda – including speakers, topics, and applications addressed at Connect 2023.

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