LI-COR is a leading innovator in developing high quality instrument systems that are used for plant research, gas analysis, drug discovery, protein research, and small animal imaging. Our instruments, analysis software, and reagents are used in more than one hundred countries—and in studies ranging from global climate change to cancer research.
LI-COR develops Environmental and Biotechnology product lines that remain at the forefront of modern research technology. Both lines continue to diversify as our scientists and engineers develop the cutting-edge products needed by researchers in today’s scientific world. Because customers are committed to pursuing challenging research, our mission is to facilitate this by providing high-quality solutions and personalized customer support.
LI-COR Biosciences has been “Impacting Lives through Science” since 1971. Building upon the strengths of our personnel, we have grown to include more than 330 employees at its headquarters in Lincoln, NE, USA, and subsidiaries in Bad Homburg, Germany, and Cambridge, UK. LI-COR is a privately held company and is ISO 9001:2015 certified.
Awards received include the Lincoln Chamber of Commerce Manufacturer of the Year Award, R&D 100 Award, Quantum Workplace Employee Voice Award for Exceptional Employee Engagement, Nebraska Exporter of Year Award, Frost and Sullivan New Product Award, Rotary Club International’s Salute to Business Award, Nebraska Business Development Council Innovation Business of the Year Award, and the Governor’s Bioscience Award. In 2021, co-founders Bill and Elaine Biggs were inducted into the Nebraska Business Hall of Fame.
In 1967, Bill graduated from the University of Nebraska-Lincoln (UNL) with a bachelor’s degree in engineering then pursued a master’s degree. At the time, the Rockefeller Foundation was funding various programs to develop grain sorghum as a food supplement in underdeveloped countries, so a grant was made on these programs’ behalf to UNL. This funding supported research initiatives for a number of scientists—including Bill—for five years and allowed UNL to sponsor a symposium at the university’s Kellogg Center.
Bill’s role in the sorghum project was to develop instrumentation related to plant photosynthesis in collaboration with other UNL scientists. One of the instruments Bill designed—an accurate, inexpensive sensor and meter for measuring photosynthetically active radiation (PAR)—was highlighted in the Journal of Ecology. Shortly after the symposium ended, his group began to receive instrumentation requests from scientists who had attended the symposium. This led to a part-time business in 1968 and to a full-time operation as Lambda Instruments Corporation in 1971. The company’s name was then formally changed to LI-COR, Inc. seven years later.
As LI-COR grew, the Environmental product line’s staff continued developing instruments to supplement the existing line of light sensors, porometers, spectroradiometers, and photosynthesis systems. The rapid growth in biotechnology research inspired LI-COR's early forays into DNA and gene sequencing technology. This eventually led to the development of a Biotechnology product line.
While the Environmental and Biotechnology product lines remain distinct, they share one common element: they measure biological parameters based on the interaction between electromagnetic radiation and physical matter.
LI-COR Environmental is a global leader in the design, manufacturing, and marketing of high quality, innovative instrument systems for plant biology and environmental research. LI-COR has been in this field for 50 years, and our instruments are used worldwide in numerous environmental applications—including agronomy, ecology, plant physiology, plant pathology, carbon cycle studies, and climate change.
Bill's master’s thesis focused on developing a light meter—much of which was later incorporated into the LI-185 Quantum/Radiometer/Photometer (right) that was marketed in 1971. At that time, light was measured primarily with foot candle meters; these meters were commonly used by photographers and measured light as the human eye perceives it (now measured with photometric sensors like the LI-210R Photometric Sensor).
However, this light measurement is not particularly useful for measuring light needed for plant growth; much of the light—particularly in the green spectrum—is reflected by plants and not used for photosynthesis. A group of researchers at the University of Wisconsin advocated to measure light in terms of photons, or the amount of light per unit of time on a unit surface area. Glass filters were used to tailor the sensor’s response to the desired quantum response (i.e., 400 to 700 nm), or the light spectrum range used by plants. This revolutionary concept ultimately led to the creation of the LI-190 Quantum Sensor in 1972 and is still sold today as the LI-190R.
LI-COR currently manufactures a complete line of terrestrial and underwater radiation sensors, as well as light meters and data loggers (i.e., the LI-250A Light Meter and LI-1500 Light Sensor Logger).
In the early 1970s, LI-COR began developing what would become a series of portable and laboratory leaf area meters. The first of these—the LI-3000 Portable Area Meter—was released in 1974. The LI-3000 combined a readout console with a scanning head for portable, non-destructive leaf area measurements. Leaf area and length as well as average and maximum width were logged by the readout console as the scanning head was drawn over a leaf. The LI-3000 was unique in that it used pulsed LEDs for the light source and a photodiode array for 1 mm2 resolution. Additionally, the LI-3050 Transparent Belt Conveyor was designed to work with the LI-3000. Both are still sold today as the LI-3000C and LI-3050C.
To add to the leaf area meter product line, LI-COR contemplated inventing a larger, laboratory grade area meter. In order to keep the instrument small enough to be practical, a camera lens and a series of mirrors are used to sense the object width. As a leaf travels under the fluorescent light source, its image is reflected by a system of three mirrors onto the scanning camera. Object width is sensed by the camera lens, and the length is provided by the current frequency as related to the rate of travel of the belt. Used in the first LI-3100 Area Meter in 1976, this system is still largely unchanged in the current LI-3100C.
In 1987, another non-destructive leaf area instrument, the LAI-2000 Plant Canopy Analyzer, was released. The LAI-2000 computed leaf area index (LAI) and other canopy structure attributes from radiation measurements made with a "fish-eye" optical sensor. Measurements made above and below the canopy are used to determine the interception of light by the canopy at 5 zenith angles. LAI is then computed with these data using a model of radiative transfer in plant canopies.
The popular LAI-2000 was updated in 2010 and released as the LAI-2200, incorporating wireless communication between the logging console and optical sensor(s). Released three years later, the current LAI-2200C added an integrated GPS module to determine solar position for light scattering corrections as well as mapping leaf area index to view on Google Earth.
In the early 1980s, LI-COR developed a portable photosynthesis system. The prototype model, which resembled a bagpipe, was among the first portable photosynthesis systems ever built and led to the release of the LI-6000 Portable Photosynthesis System.
LI-COR scientists and engineers found innovative solutions to nearly all the technical challenges of building the instrument, but one problem remained: no company manufactured an infrared gas analyzer with the performance, reliability, and low cost required for a high-quality photosynthesis system. LI-COR began developing its own infrared gas analyzer that was used in the second-generation LI-6200 photosynthesis system.
Building on the LI-6200’s success, LI-COR developed the innovative LI-6400 Portable Photosynthesis System. In addition to providing scientists with a robust and reliable instrument, the LI-6400 was developed to support many novel and custom accessories, enabling scientists to measure previously unattainable biological parameters. The LI-6400, later updated to the LI-6400XT, is the most cited photosynthesis system in published scientific journals.
In 2016, the innovative LI-6800 Portable Photosynthesis System was introduced. It delivers unprecedented capabilities for measuring gas exchange and fluorescence. Improved gas analyzer precision, light source uniformity, flow path design, environmental controls, and larger leaf areas—among other parameters—are coupled with a touch screen interface that provides real-time guidance. The LI-6800 sets a new global standard for gas exchange and fluorescence measurements in every aspect. A user experience built from the latest technological innovations—combined with integrated performance improvements—yield the most advanced system on the market.
To complement the LI-6800 and build upon the current plant physiology product line, LI-COR released the LI-600 Porometer/Fluorometer in 2020. The LI-600 is a compact, handheld device that takes stomatal conductance and chlorophyll a fluorescence measurements.
LI-COR’s infrared gas analyzer delivered higher performance at a lower cost than any other CO2 analyzer in the late 1980s. After making a few minor modifications—such as adding a sensor housing and control panel—the company marketed the LI-6251, its first standalone CO2 analyzer. This was the first-generation instrument in LI-COR's standard-setting line of infrared gas analyzers.
LI-COR infrared gas analyzers have rapidly become the world standard in atmospheric, ecological, and oceanographic research. The innovative LI-7500 Open Path CO2/H2O Analyzer, introduced in 1999, was followed by the high precision LI-7000 Enclosed CO2/H2O Analyzer a year later. The LI-7500A, an updated version of the LI-7500, was launched in 2010.
That same year LI-COR released the LI-7200—a compact, enclosed CO2/H2O analyzer that combines the benefits of open and closed path gas analyzers. Its integrated system is designed to provide measurements in harsh weather conditions and environments with low power consumption. Both the LI-7500A and the LI-7200 were updated in 2016 as the LI-7200RS and the LI-7500R, respectively; the LI-7500R was updated again to the LI-7500DS.
Advancements in gas analysis carried LI-COR into eddy covariance research technology. The eddy covariance technique is a widely used, direct method for quantifying exchanges of gases (e.g., carbon dioxide, water vapor, and methane) and energy between the earth’s surface and the atmosphere. LI-COR sought to provide a complete solution for measuring flux; it coupled its infrared gas analyzers with additional biological and meteorological sensors to measure flux over various surfaces.
In 2009, LI-COR introduced the LI-7700 Open Path CH4 Analyzer, the world's first open path methane analyzer for eddy covariance studies. As instrumentation developed, so did various software options. EddyPro® Software, for instance, was part of the creation process of the LI-7500DS and LI-7200RS. It was designed to serve as an open-source, data processing software that computes gas and energy fluxes with the eddy covariance method. These changes were also implemented in the next available software, the SmartFlux® System. SmartFlux became responsible for running EddyPro on site to compute final flux results as data are logged, providing real-time data processing.
Six years after the LI-7700 was launched, FluxSuite™ Software was offered as a way to make eddy covariance sites accessible online, so researchers could view real-time eddy covariance site results and system status. To address the post-processing stage in eddy covariance research, LI-COR then established Tovi™ Software. Tovi provides researchers with tools such as easy data visualization, gap filling, footprint modeling, and flux partitioning. FluxSuite, EddyPro, SmartFlux, and Tovi are still currently available.
Extending itself into soil flux research, LI-COR developed the LI-8100 Automated Soil CO2 Flux System, which delivers highly accurate measurements of soil CO2 flux with minimal alteration of the natural soil microclimate. The system features chambers for making both short-term survey and long-term, unattended measurements. In 2010, LI-COR released the LI-8100A, an updated and Ethernet-ready version of its predecessor.
In response to the demand for multiple chamber connections into a single system, LI-COR produced the LI-8150 Multiplexer for the LI-8100A System that connected up to sixteen long-term chambers—such as the 8100-104 Long-Term Chamber and 8100-104C Clear Long-Term Chamber. The chambers’ patented design minimize disruption and record high-precision flux measurements, creating an integrated system for soil gas flux research. All three instruments were updated and released in 2020 as the LI-8250, 8200-104, and 8200-104C, respectively.
LI-COR continued to expand its product line for soil gas flux, integrating closed path gas analyzer technology into several trace gas analyzers. For example, the LI-7810 CH4/CO2/H2O Trace Gas Analyzer, the LI-7815 CO2/H2O Trace Gas Analyzer, and the LI-7820 N2O/H2O Trace Gas Analyzer are designed for both portable and laboratory-based measurements. To assist in managing these products’ data, SoilFluxPro® Software was released and is available as a post-processing tool for fine tuning and mapping soil gas flux results.
LI-COR Biotechnology offers complete solutions for quantitative protein research and small animal imaging. From assay innovation and imaging systems to analysis software and reagents, its technology and assay protocols are used in biotechnological research around the world.
Throughout LI-COR history, LI-COR has created technology to address the growing needs of the scientific community. The Odyssey® Classic Infrared Imaging System (see “Imaging Systems”) was developed for the Western blot and quickly gained traction for its quantitative, two-color results. As this need for a highly specific and easily replicable assay continued to grow, LI-COR then invented the In-Cell Western™ Assay. This quantitative immunofluorescence assay is a valuable tool for quickly characterizing cell signaling parameters in targeted therapeutics development and for virology research. Its quick, accurate methods for detecting proteins quickly gained favor and remains a highly relevant assay within the scientific community.
After earlier developments with argon lasers, LI-COR created the Model 4000 Automated DNA Sequencer with near-infrared (NIR) laser technology, whose launch in 1992 immediately garnered international attention. This sequencer played a key role in sequencing the human genome with over a thousand base pair reads. A year later, the 4000L Long Read DNA Sequencing System set global standards for long read lengths and accuracy.
The Model 4300 DNA Analysis System was introduced in 2003. The Model 4300 System included comprehensive application packages for DNA sequencing, Microsatellite analysis, AFLP® analysis, reverse genetics (TILLING®), and SNP discovery (Ecotilling).
As the biotechnology line grew, LI-COR began to develop imaging technology; in 2001 the Odyssey Classic Infrared Imaging System was released. The Odyssey Imager delivered superior, two-color Western blots with clear, sharp bands in a fraction of the time and cost of chemiluminescence. It featured unprecedented, direct detection for a variety of applications—including Western blots, In-Cell Western Assays, and protein and DNA gels.
Three years after the Odyssey’s release, the Odyssey Sa Automated Infrared Imaging System was introduced. It was designed to work with automated microplate stacking systems to automate infrared plate-based assays, such as In-Cell Western Assays.
The Pearl® Small Animal Imager was released in 2008 and was an imperative tool for near-infrared (NIR) in vivo imaging. It enabled therapeutic companies to run in vivo assays and assess clearance, biodistribution, colocalization and drug efficacy as well as monitor and track diseases. The Pearl Trilogy was updated and reintroduced in 2015; it added a third detection channel for those researchers interested in bioluminescence detection. Using the Pearl Imager with optical agents from the LI-COR BrightSite™ line of optical agents gives researchers the ability to track biological events and disease progression in living animals. This research is valuable in numerous areas of study—including microbiomes, apoptosis, angiogenesis, inflammation, and bone growth.
The Odyssey Fc Imaging System premiered in 2010 and offers quantitative Western blot analysis with NIR and chemiluminescent detection. The Odyssey Fc Imager was intentionally designed to offer convenience to labs that use both detection methods. As with the original Odyssey Imager, it allows for NIR two-color scanning for simultaneous detection of two protein targets or the use of one channel for normalization.
One year after the Odyssey Fc Imager, LI-COR released the Odyssey CLx Infrared Imaging System. It is considered the next step in Odyssey infrared imaging technology; its AutoScan function saves time and increases the available dynamic range to an industry-leading dynamic range of greater than six logs. The Odyssey CLx Imager continues to be a flexible and multifunctional platform.
LI-COR debuted its latest innovation, the C-DiGit® Blot Scanner, in 2013. This technology offers an affordable and more accurate digital replacement for traditional, film-based methods of performing chemiluminescent Western blots. The C-DiGit Blot Scanner improves these traditional methods by collecting a single digital image with a wider dynamic range for more accurate data.
As the imaging systems were released, LI-COR also recognized the potential of infrared fluorescent dyes to improve the reliability, accuracy, and performance of DNA sequencing instruments. We soon began exploring new applications for infrared dye detection. Currently, our biotechnology line manufactures a diverse line of infrared reagents and consumables. For instance, IRDye® Infrared Dyes feature absorption and emission wavelengths in the NIR spectrum (i.e., 680 to 800 nm).
In 2010, LI-COR expanded its family of BrightSite optical agents for in vivo imaging with IRDye 800CW Infrared Dyes and IRDye 800CW EGF and 2-DG Optical Probes. Among others, these probes were developed to assist researchers in assessing disease progression. That same year, LI-COR established a translational research group to facilitate the use of its IRDye 800CW labeled imaging agents in clinical studies for detecting disease and monitoring treatment and drug efficacy. IRDye 800CW has since been conjugated to a variety of targeting agents by leading molecular imaging laboratories developing translatable agents.
As cancer clinical trials increase globally, LI-COR reagents have been an increasingly present and innovative tool—appearing in brain, lung, and breast cancer and endometriosis studies. Some IRDye infrared dyes have demonstrated potential for applications that include photodynamic and photoimmunotherapy.
To provide a complete solution for biotechnology research, LI-COR constructed software analysis programs for Western blotting. Image Studio™ Software was first developed as an industry-leading Western blot software with over 120,000 downloads as of May 2021. Then, to better align with publisher and industry best practices for better data, LI-COR then launched Empiria Studio™ Software. Empiria Studio is a post-processing, Data Integrity Software for quantitative protein expression analysis—including In-Cell Western Assays, Western blots, membranes, slides, and gels. Designed in a workflow format, it guides researchers through their experimental processes—from validation and analysis to replicability and publication. To specifically ensure that publication requirements are met by users, Empiria Studio is also created in partnership with high impact journals and incorporates the NIH Guidelines for Scientific Record Keeping.
LI-COR then launched Lambda U®, an online Western blot education portal, as a learning platform for researchers. Its courses introduce Western blotting concepts, assist in publication and grant requirements, and review laboratory techniques.
The LI-COR Board of Directors would like to take this opportunity to return thanks to God for His merciful providence in allowing LI-COR to develop and commercialize products, through the collective effort of dedicated employees that enable the examination of the wonders of His works.
"Trust in the LORD with all your heart; and do not lean on your own understanding. In all your ways acknowledge Him, and He will make your paths straight." Proverbs 3:5, 6