The LI-600N Porometer/Fluorometer comes with two default configurations preloaded: gsw+F and gsw+F+prompt. Configurations are uploaded to the instrument from the computer software (see Support: LI-600N Porometer/Fluorometer).

Figure 5‑1. Default configurations on the LI-600N.

Configuration settings

Configurations include settings for porometer and fluorometer options, measurement settings, data labels, remarks, and the instrument display layout.


General settings specify the name and author of the configuration, and the fluorometer and GPS/leaf angle options. If the fluorometer option is unchecked, the settings for the fluorometer will be hidden.

Data Labels

Data Labels are user-specified labels that can be applied to each measurement, for example genotype, plot number, or replicate. A label can be formatted as a number, list, or barcode. A configuration can have up to three labels, and at least one label must be a barcode to enable the barcode scanner on the instrument.


Remarks are optional comments that can be added to each measurement. You can create up to three remarks; one of those remarks can be applied to each measurement. Remarks are selected on the instrument when a user chooses to mark a record after taking a measurement. See Taking a measurement.

Measurement Settings

Measurement Settings control the following aspects of measurements taken with the LI-600N.

Leaf Area or Leaf Width is where you can enter values for either leaf area or leaf width, and enable a prompt to edit the leaf width during measurements.

Flow is the flow rate generated by the pump to the leaf aperture: Low (75), Medium (115), or High (150).

Match Frequency indicates how often a match of the RH sensors is triggered, specified in minutes.

Flash indicates whether the measurements are light or dark adapted, type of flash, flash intensity, and flash length in milliseconds.

Fluorescence Constants are Leaf Absorptance (abs) and Fraction Abs PSII (PS2/1).

Modulation (modrate) is the modulation rate of the LEDs used to take the fluorescence measurement. The input Actinic Modulation Rate in Hz is shown with the automatically calculated Integrated Modulation Intensity.

Display Layout

Display Layout determines the layout of the instrument display. The options include a 2- or 8-panel layout that displays selected variables after a measurement is taken.

Creating custom configurations

Custom configurations can be created in the LI-600N computer software with or without an LI-600N connected.

Go to Configuration Management from the drop-down menu in the upper left. At Local Configurations, use the green plus icon to Create New Configuration. You can also open a default configuration and save it as a new one.


Figure 5‑2. The General screen.

At the General screen, add a Configuration Name and an Author. Check Use Fluorometer; fluorometer options are hidden if unchecked. Check Use GPS and Leaf Angle to utilize the GPS receiver and accelerometer/magnetometer.

Data labels

Figure 5‑3. The Data Labels screen. This configuration would enable the barcode scanner and have a list of three labels to choose from.

In Data Labels, you can create labels using a number, list, or barcode format. One label must be a barcode to enable the barcode scanner. Each configuration can have up to three labels.

Check Prompt On Log if you want to set a data label after taking each measurement. If Prompt On Log is not checked, you set data label values prior to taking a measurement.

Enabling Prompt On Log is useful when measuring a variety of leaves that require individual labeling, for example, when screening different genotypes.

When Prompt On Log is unchecked, each label selected prior to taking a measurement will apply to subsequent measurements until a new label is chosen.

Select New Data Label. Add a Data Label Name, then select Number, List, or Barcode.

Number: Check the Auto-Increment box for automatic numbering.

List: Add up to three selections with the plus button; drag and drop them to change their order.

Barcode: Enable the barcode scanner.

Stability Criteria

Figure 5‑4. The Stability Criteria screen.

Stability criteria for fluorescence (F) and stomatal conductance (gsw) are set here. Shown are the default criteria for the LI-600N.

Table 5‑1. Stability Criteria variable options.
Preset Stability Limit (gsw) Slope Limit (F) Period (s)
Fast 0.005 5 2
Medium 0.001 1 2
Custom user specified user specified 1, 2, or 4


Remarks are optional comments that can be added to a measurement to help exclude outlier data during analysis. Select Add Remark and add text. You can create up to three remarks to choose from (e.g. "Poor clamp").

Figure 5‑5. Remarks screen.

Measurement Settings

In Measurement Settings, you can select values for Flow Rate, Leaf Area or Leaf Width, and Match Frequency for the porometer, and Flash, Fluorescence Constants, and Modulation values for the fluorometer.

Low flow is recommended for most applications to provide a measurement speed with no observed reduction in sensitivity.

Leaf Area or Leaf Width is input by the user. The default is a leaf width of 1.8 mm. Check "Prompt to edit during measurements" if you want to change the width after taking a measurement. You will be prompted after each measurement to edit the width along with any other prompts set up in the configuration (see ).

Match frequency determines how often the instrument will automatically match. Enter the interval in minutes.

Flash sets the fluorometer flash to light (default) or dark adapted, whether the flash will be rectangular or multiphase (light-adapted measurements), and sets flash intensity and flash length.

If the Dark Adapted box is checked or the Rectangular or Multiphase Flash options are selected, values in Flash and Modulation automatically set to default.

Fluorescence Constants include Leaf Absorptance and Fraction Abs PSII.

Modulation inputs the Actinic Modulation Rate. Integrated Modulation Intensity is automatically calculated.

Display Layout

In Display Layout, you can customize the layout and variables that are visible on the LI-600N Measurement screen.

Figure 5‑6. Select a 2- or 8-panel layout for the Measurement screen.

To change variables, click into the variable box then select a variable from the list. Select CLEAR to leave a variable box blank, Clear All to remove all variables, and Default to reset the layout.

All variables are recorded in the data files; this setting only indicates the variables seen in the display.


In Preview, you can view a simulation of the Measurement and Data labels screens.

Figure 5‑7. Display preview.
Figure 5‑8. Data Label preview.

Save Configuration

Select Save Configuration to save the configuration.

Upload configuration to instrument

If your instrument is not connected, go to Connection Overview from the drop-down menu and connect it. Once conneted, select the Upload button under Local Configurations to add it to the LI-600. Up to four configurations can be loaded onto the instrument at a time.