LI-6400/XT Trace Gas Sampling Kit

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The 8100-664 Trace Gas Sample Kit is designed for use with either the LI-8100A or the LI-6400/XT. This page provides assembly instructions for using the kit with the LI-6400/XT Portable Photosynthesis System.

Assembly Instructions

Tools Required: 9/16 inch or adjustable wrench

Time Required: approximately 5-10 minutes

Kit Components:

  • Lower chamber exhaust tubes (2 pieces)
  • Modified Swagelock® T-fitting
  • 6 Septa (part number 300-08998)
  • 5 sets of ¼” ferrules (2 pieces per set, part number 300-05375)
  1. Remove the brass nuts from the Swagelok® T-fitting.
  2. '
  3. Locate the lower chamber exhaust tubes for the LI-6400/LI-6400XT (two pieces, shown below) and two sets of Swagelok® 1/4 inch ferrules (two pieces per set, p/n 300-05375).
  4. Install a brass nut on each of the tubes then install one set of ferrules on each tube, as shown. Note that the orientation of the smaller piece of the ferrule set is important since it has a beveled side which seats into the large piece. When installed, the end of the tube should extend approximately 1/8 inch (3 mm) beyond the ferrules.
  5. Install the septum (p/n 300-08998) in the remaining nut. The septum has a self-sealing plastic coating on one side of the silicone disk (identified by the glossy coating). For optimum sealing, the septum should be installed with the glossy side facing the T-fitting, and the non-glossy side facing out.
  6. Assemble the T-fitting with the septum-containing nut on one “arm” of the T-fitting and short tube on the other “arm”. Install the long tube on the “leg.” Tighten the nuts, but do not over-tighten since this will create leaks around the septum and ferrules.
  7. Install the assembled Trace Gas Kit in place of the standard lower chamber exhaust tube. The ends of the tubes can be enlarged slightly with a pair of needle nose pliers (or similar) to facilitate installation on the exhaust port hose barbs of the LI-6400/ LI-6400XT head.