Configurations

The LI-600 Porometer/Fluorometer comes with four default local configurations: auto and manual modes for the porometer-only, and auto and manual modes for the porometer/fluorometer. Configurations are uploaded to the instrument from the computer software (see Support: LI-600 Porometer/Fluorometer).

Figure 5‑1. Default configurations on the LI-600.

Configuration settings

Configurations include settings for auto or manual mode, porometer and fluorometer options, measurement settings, data labels, remarks, and the instrument display layout.

General

General settings specify the name and author of the configuration, and the fluorometer option. If the fluorometer option is unchecked, the settings for the fluorometer will be hidden.

Data Labels

Data Labels are user-specified labels that can be applied to each measurement, for example genotype, plot number, or replicate. A label can be formatted as a number, list, or barcode. A configuration can have up to three labels, and at least one label must be a barcode to enable the barcode scanner on the instrument.

Automode Setup

Automode Setup enables or disables Auto Mode measurements on the instrument (see Taking a measurement in Auto Mode) and Taking a measurement in Manual Mode). Auto Mode automatically monitors stability criteria.

When Auto Mode is enabled, the LI-600 determines when to log a data point. It computes stability criteria and compares it to the settings. Once two consecutive data points are below the set threshold, the measurement is considered stable, and the LI-600 logs the data point. This is based on monitoring changes over time (in seconds) to both the computed stomatal conductance (gsw) and/or the de-modulated fluorescence signal (F).

When Auto Mode is not enabled, the instrument is in Manual Mode, and a measurement is logged by pressing the Enter button when the user's stability criteria are met.

Remarks

Remarks are optional comments that can be added to each measurement. You can create up to three remarks; one of those remarks can be applied to each measurement. Remarks are selected on the instrument when a user chooses to mark a record after taking a measurement. See Taking a measurement.

Measurement Settings

Measurement Settings control the following aspects of measurements taken with the LI-600. If the fluorometer option is not available, the fluorometer measurement settings are not be included in the configuration.

Flow is the flow rate generated by the pump to the leaf aperture: Low (75), Medium (115), or High (150).

Match Frequency indicates how often a match of the RH sensors is triggered, specified in minutes.

Flash indicates whether the measurements are light or dark adapted, type of flash, flash intensity, and flash length in milliseconds.

Fluorescence Constants are Leaf Absorptance (abs) and Fraction Abs PSII (PS2/1).

Modulation (modrate) is the modulation rate of the LEDs used to take the fluorescence measurement. The input Actinic Modulation Rate in Hz is shown with the automatically calculated Integrated Modulation Intensity.

Display Layout

Display Layout determines the layout of the instrument display. The options include a 2- or 8-panel layout that displays selected variables after a measurement is taken.

Creating custom configurations

Custom configurations can be created in the LI-600 computer software with or without an LI-600 connected.

Go to Configuration Management from the drop-down menu in the upper left. At Local Configurations, use the green plus icon to Create New Configuration. You can also open a default configuration and save it as a new one.

General

Figure 5‑2. The General configurations screen.

In General, name your configuration. First add a Configuration Name and an Author. For the LI-600 with a fluorometer, check Include Fluorometer Options; fluorometer options are hidden if unchecked. For the LI-600 with leaf angle and GPS capability, check Include GPS / Accelerometer / Magnetometer Options.

Data Labels

Figure 5‑3. The Data Labels screen.

In Data Labels, you can create labels using a number, list, or barcode format. One label must be a barcode to enable the barcode scanner. Each configuration can have up to three labels.

Check Prompt On Log if you want to set a data label after taking each measurement. If Prompt On Log is not checked, you set data label values prior to taking a measurement. Enabling Prompt On Log is useful when measuring a variety of leaves that require individual labeling, for example, when screening different genetic strains. Leave Prompt On Log unchecked to add a label or barcode to a set of measurements before taking those measurements. Each label selected prior to taking a measurement will apply to subsequent measurements until a new label is chosen.

Select New Data Label. Add a Data Label Name, then select Number, List, or Barcode.

Number: Check the Auto-Increment box for automatic numbering.

List: Add up to three selections with the plus button; drag and drop them to change their order.

Barcode: Enable the barcode scanner.

Automode Setup

In Automode Setup, select Automode, gsw, and/or F (fluorometer).

Select Automode to access the gsw and F variable options. At least one variable must be selected to enable Auto Mode.

Figure 5‑4. Automode Setup screen.

Then select the variables (gsw, F, or both), then a Preset (Fast, Medium, or Custom). If selecting Fast or Medium: the Stability Limit (gsw-specific option), Slope Limit (F-specific option), and Period over which to calculate the change are automatically determined. If selecting Custom, you can set Stability Limit, Slope Limit, and Period (1, 2, or 4 seconds). (See Table 5‑1.)

Table 5‑1. Automode Setup variable options.
Preset Stability Limit (gsw) Slope Limit (F) Period (s)
Fast 0.005 5 2
Medium 0.001 1 2
Custom user specified user specified 1, 2, or 4

To set the instrument to Manual mode, uncheck Automode. In manual mode, you set the display to view the F and gsw slopes and decide when to log the data by observing those variables on the instrument display (see Display Layout).

Remarks

In Remarks, you can create an optional comment (e.g. Poor Clamp or Chlorotic Leaf) that can be added to a measurement to help exclude outlier data during analysis. Select Add Remark and add text. You can create up to three remarks to choose from.

Figure 5‑5. Remarks setup screen.

Measurement Settings

In Measurement Settings, you can select values for Flow and Match Frequency for the porometer, and Flash, Fluorescence Constants, and Modulation for the fluorometer.

Figure 5‑6. The Measurement Settings screen.

If the Dark Adapted box is checked or the Rectangular or Multiphase Flash options are selected, values in Flash and Modulation automatically set to default.

High flow is recommended for most applications to provide the fastest measurement speed with no observed reduction in sensitivity.

Match frequency determines how often the instrument will automatically match. Enter the interval in minutes.

Flash (fluorometer) sets the flash to light (default) or dark adapted, and whether the flash will be rectangular or multiphase (light-adapted measurements), and sets flash intensity and flash length..

Fluorescence Constants (fluorometer) include Leaf Absorptance and Fraction Abs PSII.

Modulation (fluorometer). Inputs the Actinic Modulation Rate. Integrated Modulation Intensity is automatically calculated.

Display Layout

In Display Layout, you can customize the layout and variables that are visible on the LI-600 Measurement screen.

Figure 5‑7. Select a 2- or 8-panel layout for the Logged screen or the Flash Complete screen.

To change variables, click into the variable box then select the desired variable from the list. Not all variable boxes need to be filled. Select CLEAR to leave a variable box blank, Clear All to remove all variables, and Default to reset the layout.

All variables are recorded in the data files; this setting only indicates the variables seen in the display.

Preview

In Preview, you can view a simulation of the Measurement and Data labels screens.

Figure 5‑8. Display preview.
Figure 5‑9. Preview of data labels.

Save Configuration

Save Configuration to save the configuration. To load the configuration to the instrument, go to Connection Overview from the drop-down menu and connect your instrument. Select the Upload button under Local Configurations to add it to the LI-600. Up to four configurations can be loaded onto the instrument at a time.