Using the computer software

The LI-600 computer software is the interface for managing configurations and data, performing user calibrations, and for generating custom barcodes. The software can be used with or without an LI-600 connected.

Download the software and connect the LI-600

Take the following steps to download the software and connect the LI-600 to your computer.

  1. Install the computer software, which is downloaded at
  3. Press and hold the Enter button to power on the LI-600.
  4. Connect the LI-600 with your computer using the micro USB cable.
  5. The LI-600 will automatically connect with the computer software if the computer software and instrument firmware are up to date. If the software and firmware are not up to date, the computer software will prompt you to update.

Connection Overview

Connection Overview is the software startup screen, and shows the firmware and computer software versions. From here, you can proceed with or without a connected LI-600 or safely eject a connected instrument.

The software may display warning messages. See Software warning when connecting the LI-600 and Software warning when updating the firmware.

Figure 4‑1. Connection overview.

At the Connection Overview screen, you will receive one of the following prompts upon starting the computer software.

LI-600 connected! An LI-600 is powered on and has been discovered by the computer software.

  • Select Explore LI-600 to access Configuration Management, Data Management, Live View, Connection Overview, and Barcode Generator.
  • Select Offline Mode to access the Configuration Management, Data Management, Connection Overview, and Barcode Generator options that are available without an LI-600 connected.
  • Select Eject LI-600 to safely disconnect and power down the instrument.

No LI-600 detected! No LI-600 has been discovered by the computer software. Connect the instrument to the computer via USB then wait for the LI-600 connected! prompt to appear, or select Offline Mode to proceed without a connected LI-600.

Home screen

When the LI-600 is connected, select Explore LI-600 to access the Home screen. The Home screen includes a toolbar, a drop-down menu, and tiles for accessing Configuration Management, Data Management, Live View, Connection Overview, and Bar Code Generator.


The Toolbar on the home screen includes a drop-down menu in the upper left corner and three buttons in the upper right.

Drop-down menu

Figure 4‑2. The drop-down menu when an LI-600 is connected.

In addition to Configuration Management, Data Management, Live View, Connection Overview, and Bar Code Generator, the drop-down menu offers access to the following:

Instrument Calibration shows user calibration coefficients and user-adjustable parameters. Calibration parameters can be adjusted for the relative humidity (RH) sensors, flow sensors, and a fluorescence detector if there is a fluorometer. See Calibrating the sensors and User calibration procedures for instructions.

Factory Calibration appears in the drop-down menu when Allow Factory Calibration is checked in Preferences. Fluorometer, Intensity, Flow In, Flow Out, IRT, RH, and Miscellaneous values can be edited. Perform a factory reset, download a calibrations file from the LI-COR website, or perform a manual reset using a downloaded file. Select RH in the left menu to change the RH sensor serial number and to calibrate.

Preferences: select the text delimiter for data and log files (comma or tab). Check Allow Factory Calibration to enable Factory Calibration.

About Instrument displays the instrument serial number, instrument firmware version, instrument time, porometer serial number, fluorometer serial number, and the RH sensor serial number.


When an LI-600 is connected to the computer, the four buttons on the Toolbar are Home, Safely Eject Instrument, View Help, and Battery Level.

Table 4‑1. Toolbar buttons.
Home: Goes back to the home screen.

Safely Eject Instrument: Safely powers down and ejects the LI-600 from the computer software.

View Help: Goes to the LI-COR support website.

Battery Level: Displays the percent battery charge of the connected instrument.

Configuration Management

When an LI-600 is connected, the Configuration Management menu shows two configuration lists: LI-600 Configurations (those stored on the instrument) and Local Configurations (those stored on the local computer).

LI-600 Configurations

Figure 4‑3. Configurations loaded onto the LI-600.

LI-600 Configurations is the list of configurations loaded onto the connected LI-600. This section is headed with connected instrument’s serial number and organizes the data by Slot, Name, Author, Last Updated, and ID. Up to four configurations can be loaded onto the instrument at a time.

Two configurations come preloaded onto each LI-600: Manual gsw and Auto gsw for the LI-600 Porometer, and Manual gsw+F and Auto gsw+F for the LI-600 Porometer/Fluorometer. They cannot be edited but can be saved as new configurations. If a downloaded configuration has the same name as another, it will be given a randomly generated name (DL-XXX) to avoid a naming conflict. The author name LI-COR Default cannot be used for any other configurations.

Table 4‑2. Buttons to download and remove data from the instrument.

Download To Computer: Downloads the configuration to Local Configurations. Select Download on the confirmation prompt to finish.

Remove From Instrument: Removes the configuration from the LI-600. Select Remove on the confirmation prompt to finish.

Local Configurations

Figure 4‑4. Local configurations.

Local Configurations are the configurations created in the computer software. New indicates a configuration that has not been uploaded to an LI-600 since its creation or most recent edit. Double-click on a column heading to sort. Configurations with the same name as another are assigned a randomly generated name to avoid a naming conflict. Configurations can be exported and imported as PSAC files and shared among users.

Table 4‑3. Configuration management buttons.

Create New Configuration: Creates a new configuration and saves it to the Local Configurations list.

Import Configuration: Imports a configuration PSAC file.

Edit Configuration: Edits a configuration locally. Edited configurations must be re-uploaded to the LI-600.

Upload To Instrument: Uploads a configuration to LI-600. To replace a configuration, select Replace. Select Upload to complete.

Export To File: Exports the configuration as a PSAC file.

Delete Configuration: Removes the configuration from the Local Configurations list.

Data Management

Data Management includes two data lists: Data on LI-600 (data files stored on the instrument) and Local Files (data files stored in the computer software).

LI-600 data

Data on LI-600 lists the date of the last data download. This section is headed with the instrument’s serial number.

Table 4‑4. Data management buttons.

Download Instrument Data: Downloads all processed data on the LI-600 to the computer software. A copy of raw data is also downloaded as a precaution.

Clear Instrument Data: Removes all data from the LI-600.

Local Files

Figure 4‑5. Local data files.

In Local Files is a list of data and metadata stored on the computer software. You can sort the files by Config Name, Config Author, Date Taken, Porometer, Fluorometer, or Downloaded At.

Each data file includes the Number of Observations or number of measurements taken, Flash Files, Configuration Last Updated, Firmware Version, and RH Sensor Serial Number.

Table 4‑5. Data file buttons.

Export Selected Files: Downloads the selected data files to the computer’s local file system.

Toggle Selected Metadata: Shows or hides selected metadata for the selected file.

Delete Selected Files: Removes the selected data files from the Local Files.

Live View

Live View streams data from a connected LI-600 and is useful for educational settings. Live data for each selected variable in graph format are displayed, allowing you to view and log data while taking measurements.

Figure 4‑6. Live view.

To set up your parameters, select a layout from the top of the screen. Navigate through the five variable screens by selecting one of the five squares or by using the arrows. Drag and drop the desired variables into graph boxes; not all boxes need to be filled. Select CLEAR ALL to remove all variables and reset the graph boxes.

If screen does not adequately display units, select the graph titles to toggle between chart title and units.

Table 4‑6. Edit and remove variables buttons.

Edit a variable's YMin, YMax, and XSize.

Remove deletes a variable from the graph box.

When taking a measurement, data will appear in real time in the graph boxes. Using the connected LI-600, match and allow the graphs to stabilize, then take a measurement. Measurement data will appear on the computer screen and any additional connected screen.

Select Log in the upper right corner to save the last sixty minutes of data on a per-second basis to the local computer; measurement data can also be downloaded from the LI-600 in the Data Management menu at any given time. If you navigate away from the Live View menu, sixty minutes of live data are automatically saved.

Connection Overview

Connection Overview returns to the computer software startup screen.

Offline Mode

Offline Mode can be accessed with or without a connected LI-600. It includes access to Configuration Management, Data Management, Connection Overview, Bar Code Generator, and Preferences.

Figure 4‑7. Offline Mode drop-down menu.

When an LI-600 is not connected to the software, you can create and edit configurations locally, manage data that's been downloaded from the instrument, manage preferences, and create barcodes. The functionality of these features is the same as when an LI-600 is connected, with the exceptions of not being able to upload configurations or download data from the instrument.